Genetic variation of sand flies(Diptera:Psychodidae)in Gampaha and Kurunegala districts of Sri Lanka:Complementing the morphological identification

作     者：Tharaka Wijerathna Nayana Gunathilaka Wasana Rodrigo Tharaka Wijerathna;Nayana Gunathilaka;Wasana Rodrigo

作者机构：Department of ParasitologyFaculty of MedicineUniversity of KelaniyaRagamaSri Lanka Department of ZoologyFaculty of Natural SciencesThe Open University of Sri LankaNawalaNugegodaSri Lanka 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2022年第15卷第7期

页      面：322-331页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：funded by the National Research Council,Sri Lanka (Grant No. NRC 16-142) University of Kelaniya,Research Council (Grant No. RC/SROG/2021/03) 

主　　题：Morphology Sand fly Identification Barcoding Sequencing 

摘      要：Objective:To identity the variation of sand flies in the Gampaha and Kurunegala districts of Sri Lanka and to assess DNA barcoding as a complementing method for morphological ***:A total of 38441 sand flies were collected from selected localities in Gampaha and Kurunegala districts using standard entomological techniques from May 2017 to December *** were identified using morphological features and compared with mitochondrial cytochrome C oxidase subunitⅠgene-based DNA barcoding as an alternative ***:Morphological and molecular identification confirmed the presence of four species under two genera(Phlebotomus and Sergentomyia).Phlebotomus argentipes was the predominant species,followed by Sergentomyia(S.)punjabensis,*** insularis,and an unidentified Sergentomyia *** argentipes showed a clear genetic differentiation from other *** insularis and *** showed a higher genetic affinity to each other than the unidentified *** unidentified Sergentomyia species is morphologically similar to ***,but differs only in clavate ***:DNA barcoding is an effective technique for the identification of sand *** studies using molecular techniques will improve the knowledge of the cryptic diversity of Sri Lankan sand fly *** a reliable and standardized identification system for sand fly species in Sri Lanka is recommended.