Bio-inspired, bio-degradable adenosine 5′ -diphosphate-modified hyaluronic acid coordinated hydrophobic undecanal-modified chitosan for hemostasis and wound healing

作     者：Yihao Liu Haoyi Niu Chengwei Wang Xiaoxiao Yang Wentao Li Yuxin Zhang Xiaojun Ma Yuanjing Xu Pengfei Zheng Jinwu Wang Kerong Dai 

作者机构：Shanghai Key Laboratory of Orthopedic ImplantDepartment of Orthopedic SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineNo.639 Zhizaoju RdShanghai200011China Department of Orthopaedic SurgeryChildren’s Hospital of Nanjing Medical UniversityNo.72 Guangzhou RdNanjing210008China Department of RehabilitationHuashan HospitalFudan UniversityNo.12 Middle Urumqi RdShanghai200040China Med-X Research InstituteSchool of Biomedical EngineeringShanghai Jiao Tong UniversityNo.1956 Huashan RdShanghai200030China Department of OrthopedicsShanghai General HospitalShanghai Jiao Tong University School of MedicineNo.85 Wujin RdShanghai200080China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第11期

页      面：162-177页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：The study was supported by the National Key R&D Program of China(2018YFA0703000) China Postdoctoral Science Foundation(2020M681320) National Nature Science Foundation of China(grant nos.82072969) Jiangsu Provincial Key Research and Development Program(CN)(BE2019608) National Natural Science Foundation of China(82072412/81772326) Project of Shanghai Science and Technology Commission(19XD1434200/18431903700). 

主　　题：Hemostasis Antibacterial Wound healing Hyaluronic acid Chitosan 

摘      要：Uncontrolled hemorrhage and wound infection are crucial causes of trauma-associated death in both the military and the clinic. Therefore, developing an efficient and rapid hemostatic method with biocompatibility, easy degradation, and wound healing is of great importance and desirability. Inspired by spontaneous blood cell plug formation in the hemostasis process, an adenosine 5′-diphosphate modified pro-coagulation hyaluronic acid (HA-ADP) coordinated with enhanced antibacterial activity of undecanal-modified chitosan (UCS) was fabricated through physical electrostatic cross-linking and freeze-drying. The as-prepared hydrogel sponges showed a porous structure suitable for blood cell adhesion. In particular, the hydrogel exhibited excellent antibacterial ability and promoted the adhesion of platelets and red blood cells, thus inducing a prominent pro-coagulation ability via platelet activation, which exhibits a shorter hemostasis time (58.94% of control) in vitro. Compared with commercially available CELOX and gelatin sponge (GS), HA-ADP/UCS accelerates hemostasis and reduces blood loss in both rat tail amputation and rat artery injury models. Furthermore, all the samples exhibited su-perior cytocompatibility and biodegradability. Due to these performances, HA-ADP/UCS promoted full-thickness skin defect healing significantly in vivo. All the properties of HA-ADP/UCS suggest that it has great potential for translation as a clinical application material for hemostatic and wound healing.