Modular micro-PCR system for the onsite rapid diagnosis of COVID-19

作     者：Phuong Quoc Mai Nguyen Ming Wang Nelisha Ann Maria Adelicia Yongling Li Hsih Yin Tan Gordon Minru Xiong Meng-Kwang Marcus Tan Ali Asgar S.Bhagat Catherine W.M.Ong Chwee Teck Lim Phuong Quoc Mai Nguyen;Ming Wang;Nelisha Ann Maria;Adelicia Yongling Li;Hsih Yin Tan;Gordon Minru Xiong;Meng-Kwang Marcus Tan;Ali Asgar S.Bhagat;Catherine W.M.Ong;Chwee Teck Lim

作者机构：Institute for Health Innovation and Technology(iHealthtech)National University of SingaporeMD614 Medical Drive#14-01Singapore 117599Singapore Department of Biomedical EngineeringNational University of Singapore4 Engineering Drive 3Block 4#04-08Singapore 117583Singapore Advanced MedTech2 Venture Drive#23-18 Vision ExchangeSingapore 608526Singapore Infectious Diseases Translational Research ProgrammeDepartment of MedicineYong Loo Lin School of MedicineNational University of SingaporeNUHS Tower Block1E Kent Ridge Road Level 11Singapore 119228Singapore 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2022年第8卷第4期

页      面：125-136页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 

基　　金：the National University of Singapore(NUS) grant reference number NUSCOVID19RG-34 

主　　题：fir confirmation diagnosis 

摘      要：Effective containment of the COVID-19 pandemic requires rapid and accurate detection of the *** chain reaction(PCR)remains the gold standard for COVID-19 *** this article,we report the performance of a cost-effective modular microfluidic reverse transcription(RT)-PCR and RT-loop mediated isothermal amplification(RTLAMP)platform,Epidax®,for the point-of-care testing and confirmation of *** platform is versatile and can be reconfigured either for screening using endpoint RT-PCR or RT-LAMP tests or for confirmatory tests using realtime ***®is highly sensitive and detects as little as 1 RNA copy perµL for real-time and endpoint RT-PCR,while using only half of the *** achieved comparable results with those of a commercial platform when detecting SARS-CoV-2 viruses from 81 clinical RNA ***®can also detect SARS-CoV-2 from 44 nasopharyngeal samples without RNA extraction by using a direct RT-PCR assay,which shortens the sample-to-answer time to an hour with minimal user ***,we validated the technology using an RT-LAMP assay on 54 clinical RNA ***,our platform provides a sensitive,cost-effective,and accurate diagnostic solution for lowresource settings.