A surface sampling and liquid chromatography mass spectrometry method for the analysis of quaternary ammonium compounds collected from public transportation buses in New Jersey

作     者：Abigail Lazofsky Cathleen Doherty Patrick Szary Brian Buckley 

作者机构：Environmental and Occupational Health Sciences InstituteRutgers University170 Frelinghuysen RoadPiscatawayNJ08854USA Center for Advanced Infrastructure and TransportationRutgers University100 Brett RoadPiscatawayNJ08854USA 

出 版 物：《Emerging Contaminants》 (新兴污染物（英文）)

年 卷 期：2022年第8卷第1期

页      面：318-328页

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：the support of the Center for Environmental Exposures and Disease(CEED)NIH-NIEHS Grant nos.P30 ES005022 and T32 ES019854 This research was supported in part by Grant no.69A3551847102 from the U.S.Department of Transportation,Office of the Assistant Secretary for Research and Technology(OST-R) 

主　　题：Alkyl dimethyl benzyl ammonium chloride Alkyl(ethyl benzyl)dimethyl ammonium chloride Surface sampling Orbitrap mass spectrometry Public transportation COVID-19 

摘      要：Quaternary ammonium compounds(QACs)are a class of antimicrobial disinfectants whose use in cleaning products increased during the COVID-19 ***,their low vapor pressure indicates a proclivity to persist on surfaces,and their presence suggests a level of protection against *** widespread application of QACs in response to the SARS CoV-2 virus created a need to evaluate their longevity on surfaces,for both efficacy and possible health *** are however,no standardized analytical methods for QAC surface sampling and analysis,and no published studies quantifying their concentrations on mass transportation vehicles-a high occupancy,close-contact microenvironment documented to facilitate the spread the SARS CoV-2 ***,we describe a robust liquid chromatography mass spectrometry(LC-MS)method for the analysis of QACs and simultaneous development of a direct surface sampling and extraction *** demonstrate the applicability of the method through the analysis of surface samples collected from in-service public transportation *** rapid,sensitive LC-MS method included 8 target QACs quantified on a Q-Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometer using an electrospray ionization source and Dionex UltiMate 3000 UHPLC system for analyte *** standard mixtures at concentrations between 0.1 ng mL^(-1)and 2000 ng mL^(-1)were analyzed,and chromatographic separation of all analytes was achieved in less than 10 *** correlation coefficients were reported at r0.986,and LODs ranged from 0.007 to 2.103 ng mL^(-1)for all compounds,confirming the method s sensitivity.A previously reported surface sampling and extraction protocol was modified to further simplify the procedure and expand the number of target *** new sampling protocol was optimized from 10 commercially available wipes and 4 solvent types by quantifying recovery from the ***-Aid brand small gauze pads saturated with isopropanol had