L-Arginine based polyester amide/hyaluronic acid hybrid hydrogel with dual anti-inflammation and antioxidant functions for accelerated wound healing

作     者：Tong Liu Guiting Liu Jianhua Zhang Zhangfan Ding Yike Li Krishna Sigdel Xiaoyi Wang Huixu Xie Tong Liu;Guiting Liu;Jianhua Zhang;Zhangfan Ding;Yike Li;Krishna Sigdel;Xiaoyi Wang;Huixu Xie

作者机构：State Key Laboratory of Oral DiseasesNational Clinical Research Center for Oral DiseasesDepartment of Head and Neck Oncology SurgeryWest China Hospital of StomatologySichuan UniversityChengdu 610041China The State Key Laboratory of Polymer Materials EngineeringPolymer Research Institute of Sichuan UniversityChengdu 610065China College of Polymer Science and EngineeringState Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第4期

页      面：1880-1884页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (No. 52103039) Sichuan University postdoctoral interdisciplinary Innovation Fund。 

主　　题：Hydrogel Skin wound healing Antioxidant Anti-inflammation 

摘      要：Nowadays, there are still many challenges to skin regeneration. As a new type of skin substitute, hydrogel has emerging gradually with its excellent properties. However, it is still a challenge to combine with biological active agents to facilitate skin regeneration. Under the circumstance, we synthesized argininebased poly(ester amide)(Arg-PEA) and hyaluronic acid(HA-MA), and combined them into new hybrid hydrogels via photo-crosslinking. We found that the internal structure and physicochemical properties of hybrid hydrogels were greatly improved with the increase of content of Arg-PEA. Therefore, we designed hybrid hydrogels with 5 wt% and 10 wt% of Arg-PEA content, respectively. Besides, we selected the corresponding anti-inflammatory(CRP, TNF-α) indicators to detect the anti-inflammatory properties of the hybrid hydrogels at the protein level, and the corresponding antioxidant indicators(SOD, GSH/GSSG, MDA)were selected to investigate the antioxidant properties of hybrid hydrogels at the cellular level in vitro.In addition, we also selected relevant genes to test the effect of hybrid hydrogels on fibrosis and vascularization in the process of skin wound healing in vitro and verified them in vivo with a mouse dorsum wound model. The results confirmed that Arg-PEA/HA-MA(AH) hybrid hydrogel was a prospective scaffold material for skin regeneration.