Transcriptome and carotenoid profiling of different varieties of Coffea arabica provides insights into fruit color formation

作     者：Faguang Hu Xiaofei Bi Hongming Liu Xingfei Fu Yanan Li Yang Yang Xiaofang Zhang Ruirui Wu Guiping Li Yulan Lv Jiaxiong Huang Xinping Luo Rui Shi Faguang Hu;Xiaofei Bi;Hongming Liu;Xingfei Fu;Yanan Li;Yang Yang;Xiaofang Zhang;Ruirui Wu;Guiping Li;Yulan Lv;Jiaxiong Huang;Xinping Luo;Rui Shi

作者机构：Institute of Tropical and Subtropical Cash CropsYunnan Academy of Agricultural SciencesChina Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of ChinaMinistry of EducationSouthwest Forestry UniversityChina 

出 版 物：《Plant Diversity》 (植物多样性（英文版）)

年 卷 期：2022年第44卷第3期

页      面：322-334页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：financially supported by the Yunnan Science and Technology Introducing project(International Science and Technology Cooperation):Construction Project of Coffee Scientific and Technological Demonstration Districts in Mountainous Areas of Northern Laos(2019IB013) High-end Foreign Experts Program of Yunnan Thousand Talents Program(2019013) Yunnan provincial key programs(2019ZG00901,202002AA10007). 

主　　题：Coffee Carotenoids Anthocyanins Transcription factors Functional enrichment 

摘      要：The processability and ultimate quality of coffee(Coffea arabica)are determined by the composition of the matured fruits.The basis of genetic variation in coffee fruit quality could be explained by studying color formation during fruit maturation.Transcriptome profiling was conducted on matured fruits of four C.arabica varieties(orange colored fruits(ORF);purple colored fruits(PF);red colored fruits(RF)and yellow colored fruits(YF))to identify key color-regulating genes,biosynthesis pathways and transcription factors implicated in fruit color formation.A total of 39,938 genes were identified in the transcriptomes of the four C.arabica varieties.In all,2745,781 and 1224 differentially expressed genes(DEGs)were detected in YF_vs_PF,YF_vs_RF and YF_vs_ORF,respectively,with 1732 DEGs conserved among the three pairwise groups.Functional annotation of the DEGs led to the detection of 28 and 82 key genes involved in the biosynthesis of carotenoids and anthocyanins,respectively.Key transcription factors bHLH,MYB,NAC,MADS,and WRKY implicated in fruit color regulation were detected.The high expression levels of gene-LOC113688784(PSY),gene-LOC113730013(b-CHY),gene-LOC113728842(CCD7),gene-LOC113689681(NCED)and gene-LOC113729473(ABA2)in YF may have accounted for the yellow coloration.The differential expression of several anthocyanin and carotenoid-specific genes in the fruits substantially account for the purple(PF),red(RF),and orange(ORF)colorations.This study provides important insights into fruit color formation and variations in C.arabica and will help to develop coffee varieties with specific color and quality traits.