Development and evaluation of whole-genome oligonucleotide array for Acidithiobacillus ferrooxidans ATCC 23270
Development and evaluation of whole-genome oligonucleotide array for Acidithiobacillus ferrooxidans ATCC 23270作者机构:Key Laboratory of Biometallurgy of Ministry of EducationSchool of Minerals Processing and BioengineeringCentral South University
出 版 物:《中国有色金属学会会刊:英文版》 (Transactions of Nonferrous Metals Society of China)
年 卷 期:2008年第18卷第6期
页 面:1343-1351页
核心收录:
学科分类:08[工学] 0806[工学-冶金工程] 080601[工学-冶金物理化学]
基 金:Project(50621063)supported by the National Natural Science Foundation of China Project(2004CB619201)supported by the National Basic Research Program of China
主 题:寡核苷酸数列 ATCC 23270 特异性 灵敏性 量化
摘 要:为了有效地在 whole-genomic 监视 Acidithiobacillus ferrooxidans ATCC 23270 的特征,铺平,整个染色体的 50-mer-based oligonucleotide microarray 基于 A 的 3 217 ORF 被开发。ferrooxidans ATCC 23270 染色体。基于人工的 oligonucleotide 探针,结果证明最佳的杂交温度是 45 ??????? 汯杩湯 拙船樐??????