High-fidelity carbon dots polarity probes:revealing the heterogeneity of lipids in oncology
作者机构：Colllege of ChemistryInstitute of Analytical Chemistry for Life ScienceZhengzhou Key Laboratory of Functional Nanomaterial and Medical TheranosticZhengzhou University450001 ZhengzhouChina Institute of Chemical Biology and Clinical Application at the First Affiliate HospitalZhengzhou University450001 ZhengzhouChina Department of Chemistry and Chemical BiologyNortheastern UniversityBostonMA 02115USA
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2022年第11卷第7期
页 面：1555-1564页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：support from the National Natural Science Foundation of China(No.219741256) Program for Innovative Research Team(in Science and Technology)in the University of Henan Province(No.22TRTSTHN002) 111 Project of Henan Province(No.CXJD2021001) Zhonguan Scholars Program(No.202101510005)
摘 要：Polarity is an integral microenvironment parameter in biological systems closely associated with a multitude of cellular *** polarity variations accompany the initiation and development of pathophysiological ***,monitoring the abnormal polarity is of scientific and practical *** state-of-the-art monitoring techniques are primarily based on fluorescence imaging which relies on a single emission intensity and may cause inaccurate detection due to heterogeneous accumulation of the ***,we report carbon dots(CDs)with ultra-sensitive responses to *** CDs exhibit two linear relationships:one between fluorescence intensity and polarity and the other between polarity and the maximum emission *** emission spectrum is an intrinsic property of the probes,independent of the excitation intensity or probe *** features enable two-color imaging/quantitation of polarity changes in lipid droplets(LDs)and in the cytoplasm via in situ emission *** probes reveal the polarity heterogeneity in LDs which can be applied to make a distinction between cancer and normal cells,and reveal the polarity homogeneity in cytoplasm.
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