Integration of enzyme immobilization and biomimetic catalysis in hierarchically porous metal-organic frameworks for multi-enzymatic cascade reactions

作     者：Shu-Fan Li Yan Chen Yi-Shu Wang Hui-Lin Mo Shuang-Quan Zang Shu-Fan Li;Yan Chen;Yi-Shu Wang;Hui-Lin Mo;Shuang-Quan Zang

作者机构：Henan Key Laboratory of Crystalline Molecular Functional MaterialsHenan International Joint Laboratory of Tumor Theranostical Cluster MaterialsGreen Catalysis CenterCollege of ChemistryZhengzhou UniversityZhengzhou450001China School of Chemistry and Chemical EngineeringNanjing University of Science and TechnologyNanjing210094China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2022年第65卷第6期

页      面：1122-1128页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 07[理学] 08[工学] 070302[理学-分析化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：supported by the National Natural Science Foundation of China (92061201, 21825106, 22001238) the Program for Innovative Research Team (in Science and Technology) in Universities of Henan Province (19IRTSTHN022) Zhengzhou University。 

主　　题：hierarchically porous metal-organic frameworks enzyme immobilization biomimetic catalysis multi-enzymatic cascade reactions 

摘      要：Multiple enzymes-induced biological cascade catalysis is indispensable in biotechnology and industrial processes. Nevertheless,the drawbacks of most natural enzymes, including poor stability and recyclability and sensitivity to the environment, have hindered their broader application. Here, we report a facile strategy to prepare a biomimetic cascade reaction system by combining the advantages of enzyme immobilization and biomimetic catalysis in a one-pot reaction system based on the hierarchically porous metal-organic frameworks(HP-MOFs). The hierarchically porous zirconium-porphyrin-based MOF(HPPCN-222(Fe)) synthesized by modulator-induced strategy possessed tunable hierarchical porous and peroxidase-like activity,permitting them to act as not only an efficient immobilization matrix for glucose oxidase(GOx) but also peroxidase mimics to catalyze the cascade for glucose detection. A stable, anti-interference and reusable colorimetric biosensor for glucose detection was successfully established through GOx@HP-PCN-222(Fe) on the basis of the artificial tandem catalysis. Moreover, the GOx@HP-PCN-222(Fe)-fabricated electrode was available for glucose detection by electrochemical method. This work provides a potentially universal method to design functional multi-enzymatic cascade reaction systems by integrating the merits of enzyme encapsulation and biomimetic catalysis in HP-MOFs.