WRKY33-mediated indolic glucosinolate metabolic pathway confers resistance against Alternaria brassicicola in Arabidopsis and Brassica crops

作     者：Han Tao Huiying Miao Lili Chen Mengyu Wang Chuchu Xia Wei Zeng Bo Sun Fen Zhang Shuqun Zhang Chuanyou Li Qiaomei Wang Han Tao;Huiying Miao;Lili Chen;Mengyu Wang;Chuchu Xia;Wei Zeng;Bo Sun;Fen Zhang;Shuqun Zhang;Chuanyou Li;Qiaomei Wang

作者机构：Department of HorticultureZhejiang UniversityHangzhou 310058ZhejiangChina State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐productsKey Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang ProvinceInstitute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou 310021China College of HorticultureSichuan Agricultural UniversityChengdu 611130China Division of BiochemistryInterdisciplinary Plant GroupUniversity of MissouriColumbia 65211MissouriUSA State Key Laboratory of Plant GenomicsNational Centre for Plant Gene Research(Beijing)Institute of Genetics and Developmental Biologythe Chinese Academy of SciencesBeijing 100101China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第5期

页      面：1007-1019页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：supported by National Natural Science Foundation of China(32172593) the Ministry of Agriculture of China(2016ZX08009003-944001) Zhejiang Provincial Ten-Thousand Program for Leading Talents of Science and Technology Innovation(2018R52026) 

主　　题：Alternaria brassicicola glucosinolate plant defense secondary metabolism WRKY33 

摘      要：The tryptophan(Trp)-derived plant secondary metabolites,including camalexin,4-hydroxyindole-3-carbonylnitrile,and indolic glucosinolate(IGS),show broad-spectrum antifungal ***,the distinct regulations of these metabolic pathways among different plant species in response to fungus infection are rarely *** this study,our results revealed that WRKY33 directly regulates IGS biosynthesis,notably the production of 4-methoxyindole-3-ylmethyl glucosinolate(4MI3G),conferring resistance to Alternaria brassicicola,an important pathogen which causes black spot in Brassica ***33 directly activates the expression of CYP81F2,IGMT1,and IGMT2 to drive sidechain modification of indole-3-ylmethyl glucosinolate(I3G)to 4MI3G,in both Arabidopsis and Chinese kale(Brassica oleracea *** Bailey).However,Chinese kale showed a more severe symptom than Arabidopsis when infected by Alternaria *** analyses of the origin and evolution of Trp metabolism indicate that the loss of camalexin biosynthesis in Brassica crops during evolution might attenuate the resistance of crops to Alternaria *** a result,the IGS metabolic pathway mediated by WRKY33 becomes essential for Chinese kale to deter Alternaria *** results highlight the differential regulation of Trp-derived camalexin and IGS biosynthetic pathways in plant immunity between Arabidopsis and Brassica crops.