Derivatization in Liquid Chromatography/Mass Spectrometric Analysis of Neurosteroids

作     者：Kuniko MITAMURA, Kazutake SHIMADA (Faculty of Pharmaceutical Sciences, Kanazawa University 13 1, Takara machi, Kanazawa 920 0934, Japan) Kuniko MITAMURA, Kazutake SHIMADA (Faculty of Pharmaceutical Sciences, Kanazawa University 13 1, Takara machi, Kanazawa 920 0934, Japan)

作者机构：Kanazawa University 日本 9200934 

出 版 物：《色谱》 (Chinese Journal of Chromatography)

年 卷 期：2001年第19卷第6期

页      面：508-512页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 0817[工学-化学工程与技术] 08[工学] 070302[理学-分析化学] 0703[理学-化学] 

主　　题：神经类固醇 分析 液相色谱 质谱 联用技术 衍生化方法 

摘      要：Liquid chromatography/mass spectrometry (LC/MS) is now considered to be the most promising analytical method for the determination of biological substances, especially nonvolatile or highly polar substances However, some compounds do not show enough sensitivity in LC/MS and soft ionization methods commonly used in LC/MS, such as electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), sometimes do not give satisfactory structural information This report presents an overview of the derivatization methods in the LC/MS analysis of neurosteroids or neuroactive neurosteroids, which are synthesized and accumulated in the nervous system The derivatization of pregnenolone 3 sulfate, one of these steroids, with 4 ( N,N dimethylaminosulfonyl) 7 hydrazino 2,1,3 benzoxadiazole gave a satisfactory sensitivity during the quantitative analysis using LC/ESI MS The obtained results are much lower than those previously obtained using gas chromatography/mass spectrometry or radioimmunoassay On the other hand, the derivatization to acetate was useful for the treatment of labile catechol estrogens in rat brains and gave enough structural information in LC/APCI MS, which confirmed the existence of catechol estrogens in mammalian