Production of Aflatoxins from Aspergillus flavus in Liquid Medium

作     者：Maria Edite Bezerra da Rocha Francisco das Chagas Oliveira Freire Icaro Gusmao Pinto Vieira Jose Mariados Santos Filho Fabio Erlan Feitosa Maia Maria Izabel Florindo Guedes Davide Rondina 

作者机构：Universidade Estadual do Ceara Avenida Paranjana 1700 CEP: 60740-000 Fortaleza Ceara Brasil Embrapa Agroindustria Tropical Rua Dra. Sara Mesquita 2270 CEP: 60.511-110 Fortaleza-CE Brasil 

出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)

年 卷 期：2013年第7卷第4期

页      面：377-381页

学科分类：09[农学] 0903[农学-农业资源与环境] 0902[农学-园艺学] 090202[农学-蔬菜学] 

主　　题：Peanut fungi micotoxins chromatography. 

摘      要：Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus, and can be found in many grains such as peanuts, soybeans and com. This study aimed to qualitatively and quantitatively evaluate the production of aflatoxin in liquid media using strains of Aspergillus flavus obtained from peanuts marketed in the city of Fortaleza, CEo Strains of Aspergillus flavus were inoculated into a liquid medium malt extract and after 2 days inoculated into a second medium containing sucrose 5%, MgSO4·7H20 0.1%, KH2PO4 1%, ZnSO4·7H2O 0.0176 g, and cultured for 3 more days. The media were kept at room temperature ranging from 24°C to 32 °C with agitation of 130 rpm and aeration of 4.17 Llmin. Qualitative analysis was performed by thin layer chromatography and quantitatively by high performance liquid chromatography with fluorescence detection, demonstrating the production of aflatoxin B I （588 mg/L） and B2 （929 mg/L）.