Illumina Sequencing Technology as a Method of Identifying T-DNA Insertion Loci in Activation- Tagged Arabidopsis thaliana Plants

作     者：Joanna K. Polko Mohamed-Ramzi Temanni Martijn van Zanten Wilbert van Workum Sven Iburg Ronald Pierik Laurentius A.C.J. Voesenek Anton J.M. Peeters Joanna K.Polko;Mohamed-Ramzi Temanni;Martijn van Zanten;Wilbert van Workum;Sven Iburg;Ronald Pierik;Laurentius A.C.J.Voesenek;Anton J.M.Peeters

作者机构：Plant Ecophysiology Institute of Environmental Biology Utrecht University Padualaan 8 3584 CH Utrecht The Netherlands ServiceXS BV Plesmanlaan ld 2333 BZ Leiden The Netherlands Department of Cell Biology and Molecular Genetics Maryland Pathogen Research Institute (MPRI) 3128 Biosciences Research Bldg University of Maryland College Park MD 20742 USA Molecular Plant Physiology Institute of Environmental Biology Utrecht University Padualaan 8 3584 CH Utrecht The Netherlands 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2012年第5卷第4期

页      面：948-950页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 

主　　题：T-DNA 激活标签 拟南芥 测序技术 插入位点 35S启动子 识别 中国农业大学 

摘      要：Dear Editor, Forward genetic screens are commonly used as unbiased tools to isolate genes responsible for a phenotype of interest. In Arabidopsis thaliana, especially T-DNA activation tagging pop- ulations are frequently employed. These populations are gener- ated using vectors containing multiple copies of the constitutive 35S promoters derived from cau and often result in isolation i flower mosaic virus （35S CaMV） of dominant gain-of-function alleles （Weigel et al., 2000; Nakazawa et al., 2003）. This allows the study of members of large gene families that are often func- tionally redundant and, therefore, hard to identify in loss-of- function screens. Moreover, due to the dominant nature,