Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation

作     者：Xiaochan Li Boon Chin Heng Yunyang Bai Qianqian Wang Min Gao Ying He Xinwen Zhang Xuliang Deng Xuehui Zhang 

作者机构：Department of Geriatric DentistryPeking University School and Hospital of StomatologyBeijing100081PR China Department of Dental Materials&Dental Medical Devices Testing CenterPeking University School and Hospital of StomatologyBeijing100081PR China Department of ProsthodonticsPeking University School and Hospital of StomatologyBeijing100081PR China Central LaboratoryPeking University School and Hospital of StomatologyBeijing100081PR China Center of Implant DentistrySchool and Hospital of StomatologyChina Medical UniversityLiaoning Provincial Key Laboratory of Oral DiseasesShenyang110002PR China National Engineering Research Center of Oral Biomaterials and Digital Medical DevicesNMPA Key Laboratory for Dental MaterialsBeijing Laboratory of Biomedical MaterialsPeking University School and Hospital of StomatologyBeijing100081PR China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第20卷第2期

页      面：81-92页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0836[工学-生物工程] 

基　　金：supported by the National Key Research and Development Program of China(2021YFB3800800,2021YFC2400400) the National Natural Science Foundation of China(Nos.82022016,81991505,51973004,52103312) the Beijing Municipal Natural Science Foundation(7222226) Peking University Medicine Fund(PKU2020LCXQ009) 

主　　题：Surface charge Electric polarization Dose-response effect Electrical microenvironment Neurogenesis 

摘      要：Stem cells from human exfoliated deciduous teeth(SHED)uniquely exhibit high proliferative and neurogenic *** biomaterials have been demonstrated to promote neural differentiation of stem cells,but the dose-response effect of electrical stimuli from these materials on neural differentiation of SHED remains to be ***,by utilizing different annealing temperatures prior to corona poling treatment,BaTiO_(3)/P(VDF-TrFE)ferroelectric nanocomposite membranes with varying charge polarization intensity(d_(33)≈0,4,12 and 19 pC N^(-1))were *** expression of neural markers,increased cell elongation and more prominent neurite outgrowths were observed with increasing surface charge of the nanocomposite membrane indicating a dose-response effect of surface electrical charge on SHED neural *** investigations of the underlying molecular mechanisms revealed that intracellular calcium influx,focal adhesion formation,FAK-ERK mechanosensing pathway and neurogenic-related ErbB signaling pathway were implicated in the enhancement of SHED neural differentiation by surface electrical ***,this study confirms the dose-response effect of biomaterial surface charge on SHED neural differentiation and provides preliminary insights into the molecular mechanisms and signaling pathways involved.