A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis

作     者：Xing Wei Meng Yang Ze Jiang Jinhui Liu Xuan Zhang Mingli Chen Jianhua Wang Xing Wei;Meng Yang;Ze Jiang;Jinhui Liu;Xuan Zhang;Mingli Chen;Jianhua Wang

作者机构：Research Center for Analytical SciencesDepartment of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第3期

页      面：1373-1376页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financial support of the National Natural Science Foundation of China (Nos. 21727811,21922402) the Fundamental Research Funds for the Central Universities (Nos. N2005003, N2105017) the Liaoning Revitalization Talents Program (No. XLYC1802016) Scientific Research Funding Project of the Education Department of Liaoning (No. LJKZ0007)。 

主　　题：Single-cell pipette Microfluidic chip Single-cell capture ETAAS ICP-MS CdTe QDs 

摘      要：Accurate single-cell capture is a crucial step for single cell biological and chemical analysis. Conventional single-cell capturing often confront operational complexity, limited efficiency, cell damage, large scale but low accuracy, incompetence in the acquirement of nano-upgraded single-cell liquid. Flow cytometry has been widely used in large-scale single-cell detection, while precise single-cell isolation relies on both a precision operating platform and a microscope, which is not only extremely inefficient, but also not conducive to couple with modern analytical instruments. Herein, we develop a modular single-cell pipette(m SCP) microfluidic chip with high efficiency and strong applicability for accurate direct capture of single viable cell from cell suspensions into nanoliter droplets(30-1000 n L). The m SCP is used as a sampling platform for the detection of Cd Te quantum dots in single cells with electrothermal atomic absorption spectrometry(ETAAS) for the first time. It also ensures precise single-cell sampling and detection by inductively coupled plasma mass spectrometry(ICP-MS).