A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paperbased enzyme-linked immunosorbent assay

作     者：Dong Lin Bowei Li Longwen Fu Ji Qi Chunlei Xia Yi Zhang Jiadong Chen Jaebum Choo Lingxin Chen 

作者机构：CAS Key Laboratory of Coastal Environmental Processes and Ecological RemediationShandong Key Laboratory of Coastal Environmental ProcessesYantai Institute of Coastal Zone ResearchChinese Academy of Sciences264003 YantaiChina School of PharmacyBinzhou Medical University264003 YantaiChina University of Chinese Academy of Sciences100049 BeijingChina Center for Ocean Mega-ScienceChinese Academy of Sciences266071 QingdaoChina Institute of Analytical Food SafetySchool of Food Science and TechnologyJiangnan University214122 WuxiChina Department of ChemistryChung-Ang UniversitySeoul 06974South Korea 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2022年第8卷第3期

页      面：199-208页

核心收录：

学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：suppoted by Key Deployment Project of Centre for Ocean Mega-Research of Science,Chinese Academy of Sciences(COMS2019J01) the National Natural Science Foundation of China(41776110,21876066,22106179) the Shandong Provincial Natural Science Foundation Key Project(ZR2020KB022) the Shandong Provincial Natural Science Foundation Key Research and Development Project(2020CXGC010704) the National Research Foundation of Korea(2019R1A2C3004375,2020R1A5A1018052) the Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences(KLMEES202002) 

主　　题：diagnosis printing cellulose 

摘      要：Nitrocellose(NC)membranes,as porous paper-like substrates with high protein-binding capabilties,are very popular in the field of point-of-care ***,generating robust hydrophobic structures in NC membranes to fabricate microfluidic paper-based analytical devices(μPADs)remains a great *** present,the main method relies on an expensive wax printer In addition,NC membranes very easy to adhere during the printing process due to electrostatic ***,we developed a facile,fast and low-cost strategy to fabricateμPADs in NC membranes by screen-printing polyurethane acrylate(PUA)as a barrier material for defning flow channels and reaction ***,hydrophobic barriers based on UV-curable PUA can resist various surfactant solutions and organic solvents that are generally used in immunoassays and biochemical *** validate the feasibility of this PUA-based NC membrane for immunoassays in point-of-care testing(POCT),we further designed and assembled a rotational paperbased analytical device for implementing a multiplexed enzyme-linked immunosorbent assay(ELISA)in a simple *** the proposed device under the optimal conditions,alpha fetoprotein(AFP)and carcinoembryonic antigen(CEA)could be identified,with limits of detection of 136 pg/mL and 174 pg/mL,respectively,which are below the threshold values of these two cancer biomarkers for clinical *** believe that this reliable device provides a promising plaform for the diagnosis of disease based on ELISA or other related bioassays in limited setings or remote regions.