Melatonin Alleviates Liver Fibrosis by Inhibiting Autophagy
作者机构：Department of GastroenterologyFirst Affiliated Hospital of Anhui Medical UniversityKey Laboratory of Digestive Disease of Anhui ProvinceHefei 230022China Department of Critical Care Medicine of Cardiothoracic SurgeryFirst Affiliated Hospital of Anhui Medical UniversityHefei 230022China
出 版 物：《Current Medical Science》 (当代医学科学（英文）)
年 卷 期：2022年第42卷第3期
页 面：498-504页
核心收录：
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学]
基 金：Key Research and Development Project of Anhui Province(No.202004j07020039) Natural Science Research Project of Colleges and Universities in Anhui Province(No.KJ2013A155)
主 题：autophagy liver fibrosis hepatic stellate cells melatonin
摘 要：Objective Melatonin has been reported to suppress inflammation and alleviate liver fibrosis,but its effect on autophagy in liver fibrosis has not been *** study investigated the effect of melatonin on autophagy in an animal model of liver fibrosis and the hepatic stellate cell(HSC)-T6 cell *** The model was established in rats through carbon tetrachloride treatment,and melatonin was administered at three doses(2.5,5.0,and 10.0 mg/kg).Haematoxylin and eosin staining and Van Gieson’s staining were performed to examine the pathological changes of *** expression of alpha-smooth muscle actin(α-SMA)and Beclin1 in liver tissues was detected by immunohistochemical *** protein levels ofα-SMA,Beclin1 and LC3 in the animal model were detected by Western blot analysis,and gene levels of Beclin1 and LC3 were detected by quantitative real-time PCR(qRT-PCR)in the animal ***-T6 cells were activated by platelet-derived growth factor-BB(PDGF-BB).The expression ofα-SMA,Beclin1 and collagen I was detected by Western blot analysis,and the gene expression of Beclin1 and LC3 was detected by *** Melatonin reduced the expression ofα-SMA,Beclin1 and LC3 in liver *** addition,melatonin inhibited the activation of HSC-T6 cells and the expression ofα-SMA,Beclin1 and LC3 in these *** results revealed that melatonin could inhibit autophagy and HSC *** Melatonin might ameliorate liver fibrosis by regulating autophagy,suggesting that melatonin is a potential therapeutic agent for liver fibrosis.
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