Synergistic stimulation of surface topography and biphasic electric current promotes muscle regeneration
作者机构：Environmental Safety GroupKorea Institute of Science&Technology Europe(KIST-EUROPE)Saarbrücken66123Germany Department of Otorhinolaryngology-Head and Neck SurgeryKorea University College of MedicineSeoul02841Republic of Korea Department of Medical SciencesGraduate School of MedicineKorea UniversitySeoul02841Republic of Korea Center for BiomaterialsBiomedical Research InstituteKorea Institute of Science&Technology(KIST)Seoul02792Republic of Korea Laser Surface Texturing GroupAYECLUSGyeonggi-do14255Republic of Korea
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2022年第7卷第5期
页 面：118-129页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学]
基 金：supported by a Korean National Research Foundation(NRF)grant funded by the Korea government(MSIT)(No.2017M3A9G1027929) supported by both the National Research Council of Science&Technology 424(NST)grant by the Korean government(MSIP)(CAP-17-01-KIST Europe) KIST project(2E31121).
主 题：Topography Electrical field stimulation Femtosecond laser Human myogenic precursor cell Cell therapy
摘 要：Developing a universal culture platform that manipulates cell fate is one of the most important tasks in the investigation of the role of the cellular microenvironment.This study focuses on the application of topographical and electrical field stimuli to human myogenic precursor cell(hMPC)cultures to assess the influences of the adherent direction,proliferation,and differentiation,and induce preconditioning-induced therapeutic benefits.First,a topographical surface of commercially available culture dishes was achieved by femtosecond laser texturing.The detachable biphasic electrical current system was then applied to the hMPCs cultured on laser-textured culture dishes.Laser-textured topographies were remarkably effective in inducing the assembly of hMPC myotubes by enhancing the orientation of adherent hMPCs compared with flat surfaces.Furthermore,electrical field stimulation through laser-textured topographies was found to promote the expression of myogenic regulatory factors compared with nonstimulated cells.As such,we successfully demonstrated that the combined stimulation of topographical and electrical cues could effectively enhance the myogenic maturation of hMPCs in a surface spatial and electrical field-dependent manner,thus providing the basis for therapeutic strategies.
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