Dominant role of in situ native cartilage niche for determining the cartilage type regenerated by BMSCs

作     者：Mengjie Hou Baoxing Tian Baoshuai Bai Zheng Ci Yu Liu Yixin Zhang Guangdong Zhou Yilin Cao 

作者机构：Department of Plastic and Reconstructive SurgeryShanghai 9th People’s HospitalShanghai Jiao Tong University School of MedicineShanghai Key Laboratory of Tissue EngineeringShanghaiPR China National Tissue Engineering Center of ChinaShanghaiPR China Research Institute of Plastic SurgeryWei Fang Medical CollegeWeifangPR China Department of Breast SurgeryTongren HospitalShanghai Jiao Tong University School of MedicineShanghai200336PR China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第7期

页      面：149-160页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 

基　　金：This research was supported by the National Key Research and Development Program of China(2017YFC1103900) the National Natural Science Foundation of China(81671837,81871502) the Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research(2019CXJQ01) Clinical Research Plan of SHDC(SHDC2020CR2045B). 

主　　题：BMSCs Niche Cartilage type Cartilage regeneration In situ Chondrocytes 

摘      要：Tissue-engineered cartilage regeneration by bone marrow stromal cells(BMSCs)is considered an ideal method.However,how to regulate BMSCs to regenerate specific types of cartilage remains unclear,which significantly limits its clinical translation and leads to suboptimal clinical effects.Herein,we systematically explored the role of native ear and articular cartilage niches on the differentiation fate of BMSCs and the type of regenerated cartilage.First,we prepared two types of acellular cartilage sheets(ACSs)and two types of chondrocytes.Then green fluorescent protein-labeled BMSCs were seeded on two types of ACSs with or without corresponding types of chondrocytes using a sandwich model and directed or cross-implanted them into native cartilage niches.After one year of in vivo culture,cell tracking and the results of histological results showed that the native cartilage niches were capable of regulating BMSCs regeneration into specific types of cartilage that were consistent with the cartilage types of the implanted sites.Furthermore,even when the type of niche formed by ACSs or the biomimetic cartilage niche constructed by specific types of ACSs and specific types of chondrocytes did not match with the native cartilage niche,the native cartilage niche continued to determine the type of cartilage regenerated by implanted BMSCs and chondrocytes.All our results provide sufficient evidence for specific types of cartilage regeneration using chondrogenic potential cells,such as mesenchymal stem cells and chondrocytes.