Effects of electroacupuncture combined with hydrogel on the formation and changes in the glial scar in rats with spinal cord injury

作     者：Kaitan Yang Yushan Gao Yongdong Yang Chuanhong Li Zheng Cao Xiumei Wang Zaibo Li Xing Yu Kaitan Yang;Yushan Gao;Yongdong Yang;Chuanhong Li;Zheng Cao;Xiumei Wang;Zaibo Li;Xing Yu

作者机构：Ward ThreeDepartment of OrthopedicsBeijing University of Chinese Medicine Dongzhimen Hospital100010BeijingChina School of Traditional Chinese MedicineBeijing University of Chinese Medicine100029BeijingChina School of Materials Science and EngineeringTsinghua University100084BeijingChina Department of PathologyThe OHIO State University Wexner Medical CenterColumbusOH43210USA 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2022年第9卷第2期

页      面：197-205页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：financially supported by the National Natural Science Foundation of China (81973882 and 81804119) 

主　　题：Spinal cord injury Glial scar Astrocytes Electroacupuncture Jiaji acupoints Hydrogel 

摘      要：Objective: To observe the effect of electroacupuncture(EA) combined with oriented conductive bioprotein hydrogel(OCBH) on the recovery of nerve function in rats with complete spinal cord injury(SCI)and to explore its effect and mechanism on the formation and changes of glial ***: A total of 72 female Sprague-Dawley rats were randomly divided into groups according to the treatment received. A rat model of complete SCI was constructed using a spinal cord *** assessments, hematoxylin-eosin(H&E) staining, immunofluorescence staining, and Western blotting were performed at a fixed period after the ***: The material group and the material + EA group obtained better results in the behavioral assessments(all P .05) and the H&E staining. In the immunofluorescence staining and Western blotting,the GFAP protein was expressed more and denser in the material group and the material + EA group than in the model group, and the density of the GFAP expression in the material + EA group was lower at week 12 than in the material group(all P .05). The expression of complement C3 in the model, material,and material + EA groups decreased in turn. Some inflammatory factors and the NF-κB signaling pathway showed similar results in the Western blotting(all P .05). The expression of the GDNF protein in the material + EA group was significantly higher than that in the model group and the material group(both P .01).Conclusion: EA combined with OCBH can promote the recovery of motor functions after SCI by facilitating the formation of glial scars in the early stage, preventing the further spread of an inflammatory response that would affect the activation of A1/A2 astrocytes and change the morphology of glial scars at the spinal cord-material interface in its late stage.