Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol
作者机构:CAS Key Laboratory of Separation Science for Analytical ChemistryNational Chromatographic R&A CenterDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Dalian Key Laboratory of Energy BiotechnologyDalian Institute of Chemical PhysicsCAS457 Zhongshan RoadDalian116023China Division of BiotechnologyDalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian116023China University of Chinese Academy of SciencesBeijing100049China
出 版 物:《Synthetic and Systems Biotechnology》 (合成和系统生物技术(英文))
年 卷 期:2022年第7卷第3期
页 面:862-868页
核心收录:
学科分类:0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术]
基 金:supported by National Natural Science Foundation of China(22161142008,M-0246) DMTO research grant(grant no.DICP DMTO_(2)01701)from Dalian institute of Chemical Physics,CAS
主 题:Proteomics Peroxisome Methanol metabolism Systems biology
摘 要:The methylotrophic yeast Pichia pastoris(*** phaffii)has been extensively engineered for protein production,and is attracting attention as a chassis cell for methanol biotransformation toward production of small ***,the relatively unclear methanol metabolism hampers the metabolic rewiring to improve the biosynthetic *** here performed a label-free quantitative proteomic analysis of Pichia pastoris when cultivated in minimal media containing methanol and glucose,*** were 243,158 up-regulated proteins and 244,304 down-regulated proteins in log and stationary phase,respectively,when cultivated in methanol medium compared with that of glucose *** enrichment further improved the characterization of more differentially expressed proteins(481 proteins in log phase and 524 proteins in stationary phase).We demonstrated the transaldolase isoenzyme(Tal2,Protein ID:C4R244)was highly up-regulated in methanol medium cultivation,which plays an important role in methanol *** work provides important information for understanding methanol metabolism in methyltrophic yeast and will help to engineer methanol biotransformation in ***.