Four plasma membrane-localized MGR transporters mediate xylem Mg^(2+)loading for root-to-shoot Mg^(2+)translocation in Arabidopsis

作     者：Su-Fang Meng Bin Zhang Ren-Jie Tang Xiao-Jiang Zheng Rui Chen Cong-Ge Liu Yan-Ping Jing Hai-Man Ge Chi Zhang Yan-Li Chu Ai-Gen Fu Fu-Geng Zhao Sheng Luan Wen-Zhi Lan Su-Fang Meng;Bin Zhang;Ren-Jie Tang;Xiao-Jiang Zheng;Rui Chen;Cong-Ge Liu;Yan-Ping Jing;Hai-Man Ge;Chi Zhang;Yan-Li Chu;Ai-Gen Fu;Fu-Geng Zhao;Sheng Luan;Wen-Zhi Lan

作者机构：State Key Laboratory for Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210023JiangsuChina Institute of Future AgricultureNorthwest A&F UniversityYangling 712100ShaanxiChina The Key Laboratory of Western Resources Biology and Biological TechnologyCollege of Life SciencesNorthwest UniversityXi'an 710069China Department of Plant and Microbial BiologyUniversity of CaliforniaBerkeleyCA 94720USA 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2022年第15卷第5期

页      面：805-819页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China(31770267) the National Science Foundation(MCB-1715764) 

主　　题：functional conservation metal nutrient magnesium long-distance transport phenotyping 

摘      要：Magnesium(Mg^(2+)),an essential structural component of chlorophyll,is absorbed from the soil by roots and transported to shoots to support photosynthesis in ***,the molecular mechanisms underlying root-to-shoot Mg^(2+)translocation remain largely *** describe here the identification of four plasma membrane(PM)-localized transporters,named Mg^(2+)release transporters(MGRs),that are critical for root-to-shoot Mg transport in *** complementation assays in a Mg^(2+)-uptake-defi-cient bacterial strain confirmed that these MGRs conduct Mg^(2+)***-localized MGRs(MGR4,MGR5,MGR6,and MGR7)were expressed primarily in root stellar cells and participated in the xylem loading step of the long-distance Mg^(2+)transport *** particular,MGR4 and MGR6 played a major role in shoot Mg homeostasis,as their loss-of-function mutants were hypersensitive to low Mg^(2+)but tolerant to high Mg^(2+)*** grafting analysis further demonstrated that MGR4 functions in the root to determine shoot Mg^(2+)accumulation and physiological phenotypes caused by both low-and high-Mg^(2+)*** together,our study has identified the long-sought transporters responsible for root-to-shoot Mg^(2+)translocation in plants.