Yes-associated protein contributes to magnesium alloy-derivedinflammation in endothelial cells

作     者：Hongchi Yu Zhe Hou Nuoya Chen Rifang Luo Li Yang Michael Miao Xiaoyi Ma Lifeng Zhou Fugui He Yang Shen Xiaoheng Liu Yunbing Wang 

作者机构：National Engineering Research Center for BiomaterialsSichuan UniversityChengdu 610065China Institute of Biomedical EngineeringWest China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu 610041China Division of Oral&Craniofacial Health SciencesUniversity of North Carolina Adams School of DentistryChapel HillNC 27599USA Beijing Key Laboratory of Cardiac Drug Device Technology and Evidence Based MedicineBeijing 100021China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2022年第9卷第1期

页      面：130-142页

核心收录：

学科分类：08[工学] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：supported by the National Natural Science Foundation of China(11802190) National Key Research and Development Program(2016YFC1102200) the 111 Project The Program of Introducing Talents of Discipline to Universities(B16033) 

主　　题：magnesium alloy Yes-associated protein inflammation 

摘      要：Magnesium alloy(Mg alloy)has attracted massive attention in the potential applications of cardiovascular stents because of its good biocompatibility and ***,whether and how the Mg alloy induces inflammation in endothelial cells remains *** the present work,we investigated the activation of Yes-associated protein(YAP)upon Mg alloy stimuli and unveiled the transcriptional function in Mg alloy-induced *** RT–PCR,western blotting and immunofluorescence staining showed that Mg alloy inhibited the Hippo pathway to facilitate nuclear shuttling and activation of YAP in human coronary artery endothelial cells(HCAECs).Chromatin immunoprecipitation followed sequencing was carried out to explore the transcriptional function of YAP in Mg alloy-derived *** led to the observation that nuclear YAP further bonded to the promoter region of inflammation transcription factors and co-transcription *** binding event activated their transcription and modified mRNA methylation of inflammation-related genes through regulating the expression of N6-methyladenosine modulators(METTL3,METTL14,FTO and WTAP).This then promoted inflammation-related gene expression and aggravated inflammation in *** YAP deficiency cells,Mg alloy-induced inflammation was ***,our data suggest that YAP contributes to the Mg alloy-derived inflammation in HCAECs and may provide a potential therapeutic target that alleviates inflammation after Mg alloy stent implantation.