EGCG and ECG induce apoptosis and decrease autophagy via the AMPK/mTOR and PI3K/AKT/mTOR pathway in human melanoma cells

作     者：DU Bing-Xin LIN Pei LIN Jun DU Bing-Xin;LIN Pei;LIN Jun

作者机构：School of Life Sciences and Health EngineeringJiangnan UniversityWuxi 214122China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2022年第20卷第4期

页      面：290-300页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China(No.82004027) the Fundamental Research Funds for the Central Universities(JUSRPI1961) 

主　　题：EGCG ECG Apoptosis Autophagy PI3K/AKT/mTOR 

摘      要：Catechins have been proven to exert antitumor effects in different kinds of ***,the underlying mechanisms have not been completely clarified *** study aimed to assess the effects and mechanisms of(-)-epigallocatechin-3-gallate(EGCG)and(-)-epicatechin-3-gallate(ECG)on human melanoma skin A375 *** showed that EGCG and ECG inhibited the proliferation of A375 cells and ECG showed better inhibitory *** cytometry analysis had shown that EGCG and ECG induced apoptosis and led to cell cycle *** and ECG decreased Bcl-2 expression and upregulated Caspase-3 protein level,indicating the development of ***,EGCG and ECG could decreased mitochondrial membrane potential of A375 *** addition,the expression of Beclin-1,LC3 and Sirt3 were downregulated at protein levels,which known to be associated with *** autophagy was increased by rapamycin,the apoptotic trend was not change,indicating that apoptosis and autophagy are ***,EGCG and ECG treatments decreased phosphorylated-AMPK(p-AMPK)and increased the ratios of P-PI3K,p-AKT and p-mTOR in melanoma ***,EGCG and ECG induced apoptosis via mitochondrial signaling pathway,downregulated autophagy through modulating the AMPK/mTOR and PI3K/AKT/mTOR signaling *** indicated that EGCG and ECG may be utilized in human melanoma treatment.