Mapping of Sentinel Lymph Node Micrometastasis in Dukes-B Colorectal Carcinoma and Its Clinical Significance

作     者：Xiongfei Yang Tao Wang Weisheng Zhang Lili Feng Binbin Du Aili Zhang 

作者机构：Department of Colorectal Surgery Gansu People's Hospital Lanzhou 730000 Gansu Province. China. 

出 版 物：《Clinical oncology and cancer researeh》 (CLINICAL ONCOLOGY AND CANCER RESEARCH)

年 卷 期：2009年第6卷第5期

页      面：347-353页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 071010[理学-生物化学与分子生物学] 100705[医学-微生物与生化药学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071005[理学-微生物学] 10[医学] 

基　　金：supported by a grant from Gansu Provincial Program for Tackling Key Problems in Science and Technology China (No.2GS064-B43-020-36) 

主　　题：colorectal carcinoma sentinel lymph node micrometastasis. 

摘      要：OBJECTIVE To investigate the detection of sentinel lymphnode (SLN) micrometastasis (MM) and the clinical significancein patients with Dukes-B colorectal carcinoma (CRC) using H&Estained *** In a prospective study of 64 patients with Dukes-BCRC undergoing radical surgery,routine H&E staining combinedwith the immunohistochemical SP method was used to detect theexpression of the cytokeratin 20 (CK20) and telomerase (TLMA)in 122 SLNs from 61 of the 64 *** a 3-year follow up,the patients clinicopathologic parameters data,survival and theirrelationship were collected and *** i) In 6 out of the 61 cases with successful mapping,9SLNs were positive after routine detection using H&E ***) In the other 55 with negative results from H&E staining,thepositive rate of CK20 expression was 27.3% (15/55),and thepositive rate of TLMA 21.8% (12/55),after immunohistochemicalexamination (IHC) on the ***) Detection by combining the 2methods showed that the micrometastasis rate was 38.2% (21/55).iv) The recurrence or metastasis rate of CRC was significantlyhigher in the Dukes-B patients with sentinel lymph nodemicrometastasis (SLNMM) (+) than in those with SLNMM (-)(P0.05),and simultaneously,the survival rate decreased by alarge margin in the patients with SLNMM (+),compared to thosewith SLNMM (-) (P0.05).There were significant differences inthe recurrence or metastasis rate of cancer and the survival ratebetween the Dukes-B patients with SLNMM (-) and the Dukes-Cpatients (P0.05).CONCLUSION Examination of anti-CK20 and anti-TLMA usingimmunohistochemical staining can be used to detect SLNMM,andthe combined examination of the 2 can increase the detection *** detection of SLNMM can precisely determine the Dukes stageof colorectal carcinoma,which is of help in directing postoperativeadjunctive therapy and in assessing prognosis.