Genetic diversity and population structure of Robinia pseudoacacia from six improved variety bases in China as revealed by simple sequence repeat markers

作     者：Qi Guo Sen Cao Li Dong Xiuyu Li Jiangtao Zhang Yuanshuai Zhang Zijie Zhang Yuhan Sun Cui Long Yingming Fan Chao Han Peiyao Han Xiaodi Liu Yun Li Qi Guo;Sen Cao;Li Dong;Xiuyu Li;Jiangtao Zhang;Yuanshuai Zhang;Zijie Zhang;Yuhan Sun;Cui Long;Yingming Fan;Chao Han;Peiyao Han;Xiaodi Liu;Yun Li

作者机构：Beijing Advanced Innovation Center for Tree Breeding By Molecular DesignEngineering Technology Research Center of Black Locust of National Forestry and Grassland AdministrationCollege of Biological Sciences and TechnologyNational Engineering Laboratory for Tree BreedingBeijing Forestry UniversityBeijing 100083P.R.China College of AgricultureHenan University of Science and TechnologyLuoyang 471023P.R.China Henan Academy of ForestryZhengzhou 450008P.R.China Feixian County State Owned Daqingshan Forest FarmLinyi 273400P.R.China 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2022年第33卷第2期

页      面：611-621页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0907[农学-林学] 08[工学] 0829[工学-林业工程] 0901[农学-作物学] 

基　　金：This work was supported by the Doctoral Scientific Research Starting Foundation of Henan University of Science and Technology(13480089) National Nature Science Foundation of China(31570677) the National Key R&D Program of China(2017YFD0600503) the National Forestry and Grassland Administration of Science and Technology Development Center Project(2016007). 

主　　题：Robinia pseudoacacia Genetic diversity Genetic differentiation Microsatellite Population structure Black locust 

摘      要：Robinia pseudoacacia is an important afforesta-tion tree introduced to China in 1878.In the present study,we examined the genetic diversity among 687 strains rep-resenting four improved varieties and two secondary prov-enances,comprising 641 clones and 46 seedlings.Ninety-one simple sequence repeats(SSRs)were selected through segregation analysis and polymorphism characterization,and all sampled individuals were genotyped using well-characterized SSR markers.After excluding loci with non-neutral equilibrium,missing locus data and null alleles,we used 36 primer pairs to assess the genetic diversity of these germplasm resources,revealing vast genetic differentiation among the samples,with an average of 8.352 alleles per locus and a mean Shannon′s index of 1.302.At the popula-tion level,the partitioning of variability was assessed using analysis of molecular variance,which revealed 93%and 7%variation within and among collection sites,respectively.Four clusters were detected using structure analysis,indi-cating a degree of genetic differentiation among the six populations.Insights into the genetic diversity and structure of R.pseudoacacia provide a theoretical basis for the con-servation,breeding and sustainable development in China.