Decoupling the role of chemistry and microstructure in hMSCs response to an osteoinductive calcium phosphate ceramic
作者机构：MERLN Institute for Technology Inspired Regenerative MedicineMaastricht Universitythe Netherlands Kuros Biosciences BV3723 MBBilthoventhe Netherlands
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2023年第19卷第1期
页 面：127-138页
核心收录：
学科分类：08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学]
基 金：the support of the Dutch Province of Limburg(LINK project) the Gravitation Program "Materials Driven Regeneration" funded by the Dutch Research Council NWO
主 题：Calcium phosphate Microstructure Chemical composition Mesenchymal stromal cells Osteogenic differentiation
摘 要：Calcium phosphates(CaP)are widely used synthetic bone graft substitutes,having bioactivity that is regulated by a set of intertwined physico-chemical and structural *** some CaPs have shown to be as effective in regenerating large bone defects as autologous bone,there is still the need to understand the role of individual material properties in CaP ***,we aimed to decouple the effects of chemical composition and surface-microstructure of a beta-tricalcium phosphate(TCP)ceramic,with proven osteoinductive potential,on human mesenchymal stromal cells(hMSCs)*** this end,we replicated the surface structure of the TCP ceramic into polylactic acid without inorganic additives,or containing the chemical constituents of the ceramic,i.e.,a calcium salt,a phosphate salt,or TCP *** microstructure of the different materials was characterized by confocal laser *** were cultured on the materials,and the expression of a set of osteogenic genes was *** cell culture medium was collected and the levels of calcium and phosphate ions were quantified by inductively-coupled plasma mass *** results revealed that none of the tested combinations of properties in polymer/composite replicas was as potent in supporting the osteogenic differentiation of hMSCs as the original ***,we observed some effects of the surface structure in the absence of inorganics,as well as combined effects of surface structure and the added salts,in particular calcium,on osteogenic *** approach presented here can be used to study the role of independent properties in other CaP-based biomaterials.
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