A facile and general method for synthesis of antibiotic-free protein-based hydrogel:Wound dressing for the eradication of drug-resistant bacteria and biofilms

作     者：Jiang Ouyang Qingyue Bu Na Tao Mingkai Chen Haijun Liu Jun Zhou Jinggong Liu Bo Deng Na Kong Xingcai Zhang Tianfeng Chen Yihai Cao Wei Tao 

作者机构：The First Affiliated HospitalDepartment of ChemistryJinan UniversityGuangzhou510632China Center for Nanomedicine and Department of AnesthesiologyBrigham and Women’s HospitalHarvard Medical SchoolBostonMA02115USA John A.Paulson School of Engineering and Applied SciencesHarvard UniversityCambridgeMA02138USA College of Chemistry and Chemical EngineeringCentral South UniversityChangshaHunan410083China The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangdong Provincial Academy of Chinese Medical SciencesGuangzhou510006China Department of MicrobiologyTumor and Cell BiologyKarolinska InstituteStockholm17177Sweden 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第12期

页      面：446-458页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：Harvard Medical School/Brigham and Women’s Hospital Department of Anesthesiology-Basic Scientist Grant(No.2420 BPA075,W.T.) Center for Nanomedicine Research Fund(No.2019A014810,W.T.) Gillian Reny Stepping Strong Center for Trauma Innovation Breakthrough Innovator Award(No.113548,W.T.) Nanotechnology Foundation(No.2022A002721,W.T.) Farokhzad Family Distinguished Chair Foundation(W.T.) Khoury Innovation Award(No.2020A003219,W.T.) and American Heart Association(AHA)Collaborative Sciences Award(No.2018A004190,W.T.) .J.O.was supported by the China Postdoctoral Science Foundation(No.2020M683173) 

主　　题：THPS Protein hydrogel Bacterial resistance Anti-Biofilm Wound dressing 

摘      要：Antibacterial protein hydrogels are receiving increasing attention in the aspect of bacteria-infected-wound healing. However, bacterial drug resistance and biofilm infections lead to hard healing of wounds, thus the construction of biological agents that can overcome these issues is essential. Here, a simple and universal method to construct antibiotic-free protein hydrogel with excellent biocompatibility and superior antibacterial activity against drug-resistant bacteria and biofilms was developed. The green industrial microbicide tetrakis (hydroxymethyl) phosphonium sulfate (THPS) as cross-linking agent can be quickly cross-linked with model protein bovine serum albumin (BSA) to form antibacterial hydrogel through simple mixing without any other initiators, subsequently promoting drug-resistance bacteria-infected wound healing. This simple gelatinization strategy allows at least ten different proteins to form hydrogels (e.g. BSA, human serum albumin (HSA), egg albumin, chymotrypsin, trypsin, lysozyme, transferrin, myohemoglobin, hemoglobin, and phycocyanin) under the same conditions, showing prominent universality. Furthermore, drug-resistance bacteria and biofilm could be efficiently destroyed by the representative BSA hydrogel (B-Hydrogel) with antibacterial activity, overcoming biofilm-induced bacterial resistance. The in vivo study demonstrated that the B-Hydrogel as wound dressing can promote reepithelization to accelerate the healing of methicillin-resistant staphylococcus aureus (MRSA)-infected skin wounds without inducing significant side-effect. This readily accessible antibiotic-free protein-based hydrogel not only opens an avenue to provide a facile, feasible and general gelation strategy, but also exhibits promising application in hospital and community MRSA disinfection and treatment.