CFPU: A Cell-Free Processing Unit for High-Throughput, Automated In Vitro Circuit Characterization in Steady-State Conditions
作者机构：Institute of BioengineeringSchool of EngineeringÉcole Polytechnique Fédérale de LausanneSwitzerland
出 版 物：《BioDesign Research》 (生物设计研究（英文）)
年 卷 期：2021年第2021卷第1期
页 面：1-11页
学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学]
主 题：reagent sustained automation
摘 要：Forward engineering synthetic circuits are at the core of synthetic *** solutions will be required to facilitate circuit design and *** design is increasingly being automated with design software,but innovations in experimental automation are lagging *** technologies made it possible to perform in vitro transcription-translation(tx-tl)reactions with increasing throughput and sophistication,enabling screening and characterization of individual circuit elements and complete circuit ***,we developed an automated microfluidic cell-free processing unit(CFPU)that extends highthroughput screening capabilities to a steady-state reaction environment,which is essential for the implementation and analysis of more complex and dynamic *** CFPU contains 280 chemostats that can be individually programmed with DNA *** chemostat is periodically supplied with tx-tl reagents,giving rise to sustained,long-term steady-state *** microfluidic pulse width modulation(PWM),the device is able to generate tx-tl reagent compositions in real *** device has higher throughput,lower reagent consumption,and overall higher functionality than current chemostat *** applied this technology to map transcription factor-based repression under equilibrium conditions and implemented dynamic gene circuits switchable by small *** expect the CFPU to help bridge the gap between circuit design and experimental automation for in vitro development of synthetic gene circuits.
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