Real-time quantification of nuclear RNA export using an intracellular relocation probe

作     者：Jie Shen Juan Chen Dong Wang Zhengjie Liu Guangmei Han Bianhua Liu Mingyong Han Ruilong Zhang Guodong Liu Zhongping Zhang Jie Shen;Juan Chen;Dong Wang;Zhengjie Liu;Guangmei Han;Bianhua Liu;Mingyong Han;Ruilong Zhang;Guodong Liu;Zhongping Zhang

作者机构：School of Chemistry and Chemical EngineeringInformation Materials and Intelligent Sensing Laboratory of Anhui Provinceand Institute of Physical Science and Information TechnologyAnhui UniversityHefei 230601China Key Laboratory of Structure and Functional Regulation of Hybrid Materials(Anhui University)Ministry of EducationHefei 230601China Key Lab of Photovoltaic and Energy Conservation MaterialsInstitute of Solid State PhysicsHFIPSChinese Academy of SciencesHefei 230031China School of Life and Health SciencesAnhui Science and Technology UniversityChuzhou 233100China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第8期

页      面：3865-3868页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.21775001,21874137,21974001,21976183and 22074001) Natural Science Foundation of Anhui Province(No.1808085MB32) Nature and Science Foundation from Anhui Province Ministry of Education(No.KJ2019A0011) 

主　　题：RNA export Quantification Relocation probe Fluorescence imaging Hormone 

摘      要：Nuclear RNA export into the cytoplasm is one of the key steps in protein expression to realize biological *** the broad availability of nucleic acid dyes,tracking and quantifying the highly dynamic process of RNA export in live cells is *** dye-labeled RNA enters the cytoplasm,the dye molecules are released upon degradation of the RNA,allowing them to re-enter the cell *** a result,the ratio between the dye exported with RNA into the cytoplasm and the portion staying inside the nucleus cannot be *** address this common limitation,we report the design of a smart probe that can only check into the nucleus *** adding to cells,this probe rapidly binds with nuclear RNAs in live cells and reacts with intrinsic H_(2)*** reaction not only activates the fluorescence for RNA tracking but also changes the structure of probe and consequently its intracellular *** disassociating from exported RNAs in cytoplasm,the probe preferentially enters lysosomes rather than cell nucleus,enabling real-time quantitative measurement of nuclear RNA *** this probe,we successfully evaluated the effects of hormones and cancer drugs on nuclear RNA export in live ***,we found that hormones inhibiting RNA exports can partially offset the effect of chemotherapy.