Hypoxia induced apoptosis of rat gastric mucosal cells by activating autophagy through HIF-1α/TERT/mTORC1 pathway

作     者：YAPING WANG XIAOHONG XUE ZHEN ZHAO XIAOLIN LI ZHIYONG ZHU 

作者机构：Department of Digestive MedicineWuxi Huishan District People’s HospitalWuxiChina Department of Digestive MedicineQinghai Provincial People’s HospitalXiningChina 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2022年第46卷第7期

页      面：1651-1659页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This study is supported by the National Natural Science Foundation of China(No.81760334) Innovative and Entrepreneurial Doctor Program of Jiangsu Provincial,Top Talents Support Program for Young and Middle-Aged People of Wuxi Health Committee and The Scientific Research Project of Wuxi Health committee(Youth Project,No.Q201912).The funds have no role in the design of the study,the collection,analysis,and interpretation of data,and the composition of the manuscript 

主　　题：Hypoxia Autophagy Apoptosis HIF-1α TERT mTORC1 

摘      要：The pathogenesis of high altitude-related gastric mucosal injury remains poorly understood,this study aimed to investigate the role of autophagy in hypoxia-induced apoptosis of rat gastric mucosal *** were randomized into four groups which were maintained at an altitude of 400 m(P)or received no treatment(H),autophagy inducer rapamycin(H+AI)or autophagy inhibitor 3-MA(H+AB)at an altitude of 4,300 m for 1,7,14 and 21 days,respectively,and the morphology,ultrastructure,autophagy,and apoptosis of gastric mucosal tissues were *** mucosal epithelial cells CC-R039 were cultured under conditions of normoxia,2%O2(hypoxia),or 2%O2+anti-mTORC1 for 0,24,48,and 72 h,respectively,and the autophagy and apoptosis were ***-R039 cells were transfected with siHIF-1α,siTERT,or siRNA and the autophagy was *** results showed that the exposure to hypoxia increased the autophagy and apoptosis of gastric mucosal cells in rats,and apoptosis was aggravated by rapamycin treatment but alleviated by 3-MA *** duration of hypoxia from 0 to 72 h could increase the autophagy and apoptosis but decrease the proliferation of gastric mucosal *** of mTORC1 with rapamycin led to further increase in apoptosis and even substantial cell death,and inhibition of HIF-1αand TERT increased mTORC1 expression and reduced ***,the inhibition of HIF-1αreduced TERT *** conclusion,hypoxia could induce apoptosis of rat gastric mucosal cells by activating autophagy through HIF-1α/TERT/mTORC1 pathway.