Expression of Shiga Toxin B Subunit at Cell Surface in E. coli K-12

作     者：苏国富 Himanshu N.Brahmbhatt Kenneth N.Timmis 

作者机构：Institute of Biotechnology Academy of Military Medical Sciences CPLA Beijing 100850 PRC National Research Centre for Biotechnology Germany 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：1993年第36卷第11期

页      面：1352-1360页

核心收录：

学科分类：0810[工学-信息与通信工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0708[理学-地球物理学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0704[理学-天文学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

主　　题：Shiga toxin B subunit LamB gene E. coli K-12 cell surface expression. 

摘      要：The three parts(Stx17B, Stx27B and StxB) of Shiga toxin B subunit have been fused into a cell surface exposed loop of the LamB protein at a BamH I site between residues 153 and 154. Western blotting revealed that the three parts of Shiga toxin B subunit could be expressed as the Lamb fusion proteins in E. coli. Indirect immunofluorescence and immunoelectron microscopy analyses showed fusion proteins LamB/Stx17B and LamB/Stx27B could be expressed at cell surface in E. coli, but fusion protein LamB/StxB could not be expressed at cell surface; it was aggregated in cytoplasm and was toxic to host. This expression system provided a new way to construct an oral live vaccine against Shigella dysenteriae 1.