A single nucleotide mutation in the dual-oxidase 2(DUOX2) gene causes some of the panda's unique metabolic phenotypes

作     者：Agata M.Rudolf Qi Wu Li Li Jun Wang Yi Huang Jacques Togo Christopher Liechti Min Li Chaoqun Niu Yonggang Nie Fuwen Wei John R.Speakman Agata M.Rudolf;Qi Wu;Li Li;Jun Wang;Yi Huang;Jacques Togo;Christopher Liechti;Min Li;Chaoqun Niu;Yonggang Nie;Fuwen Wei;John R.Speakman

作者机构：State Key Laboratory of Molecular Development Institute of Genetics and Developmental Biology Chinese Academy of Sciences Institute of Zoology Chinese Academy of Sciences Institute of Microbiology Chinese Academy of Sciences Institute of Biological and Environmental Sciences University of Aberdeen Centre of Excellence for Animal Ecology and Genetics Chinese Academy of Sciences Shenzhen Key Laboratory of Metabolic Health Center for Energy Metabolism and Reproduction Shenzhen Institute of Advanced Technology Chinese Academy of Sciences 

出 版 物：《National Science Review》 (国家科学评论(英文版))

年 卷 期：2022年第9卷第2期

页      面：34-42页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB13030100 and XDB29020000) the Creative Research Group Project of National Natural Science Foundation of China (31821001) the Key Project of the Chinese Academy of Sciences (QYZDB-SSWSMC047) the National Key Research and Development Program of China (2018YFC2000500) the Chinese Academy of Sciences President’s International Fellowship Initiative Postdoctoral Fellowship (to A.M.R.) the President’s International Fellowship Initiative Professorial and Wolfson Merit Award (to J.R.S.) 

主　　题：giant panda DUOX2 mutation metabolic rate thyroid hormones DUOX2 mice and microbiota 

摘      要：The giant panda(Ailuropoda melanoleuca) is an iconic bear native to China,famous for eating almost exclusively *** unusual dietary behavior for a carnivore is enabled by several key adaptations including low physical activity,reduced organ sizes and hypothyroidism leading to lowered energy *** adaptive phenotypes have been hypothesized to arise from a panda-unique single-nucleotide mutation in the dual-oxidase 2(DUOX2) gene,involved in thyroid hormone *** test this hypothesis,we created genome-edited mice carrying the same point mutation as the panda and investigated its effect on metabolic *** mice were 27% smaller than heterozygous and wild-type ones,had 13 % lower body mass-adjusted food intake,5 5 % decreased physical activity,lower mass of kidneys(11%) and brain(5%),lower serum thyroxine(T4:36%),decreased absolute(12%) and mass-adjusted(5%) daily energy expenditure,and altered gut *** with T4 reversed the effects of the *** work uses a state-of-the-art genome editing approach to demonstrate the link between a single-nucleotide mutation in a key endocrine-related gene and profound adaptive changes in the metabolic phenotype,with great importance in ecology and evolution.