circSVIL regulates bovine myoblast development by inhibiting STAT1 phosphorylation

作     者：Binglin Yue Haiyan Yang Jiyao Wu Jian Wang Wenxiu Ru Jie Cheng Yongzheng Huang Xianyong Lan Chuzhao Lei Hong Chen Binglin Yue;Haiyan Yang;Jiyao Wu;Jian Wang;Wenxiu Ru;Jie Cheng;Yongzheng Huang;Xianyong Lan;Chuzhao Lei;Hong Chen

作者机构：Key laboratory of Animal GeneticsBreeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第2期

页      面：376-386页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：the National Natural Science Foundation of China(31772574)。 

主　　题：circSVIL STAT1 skeletal muscle 

摘      要：Circular RNAs(circRNAs),a novel class of non-coding RNAs with a loop structure,have recently been shown to participate in various pathophysiological processes.However,the precise role of circRNAs in myoblasts remains unclear.In this report,circSVIL was screened and identified from our previous sequencing analysis;we then performed gain-and loss-of-function experiments on bovine myoblasts by CCK8,EdU,flow cytometry,qRT-PCR,and Western blotting.The results indicate that circSVIL facilitates bovine myoblast proliferation and inhibits cell apoptosis.Using mechanism assays such as bioinformatics prediction,RNA immunoprecipitation(RIP),and cytoplasmic separation,we demonstrate that circSVIL could interact with STAT1 and inhibit STAT1 phosphorylation,thereby restraining STAT1’s nuclear translocation and affecting its downstream signal cascade.Our results may elucidate a new regulatory pathway for bovine skeletal muscle development.