In Silico search and biological validation of MicroR171 family related to abiotic stress response in mulberry(Morus alba)

作     者：Zhichao Sun R.M.Saravana Kumar Jisheng Li Guiming Yang Yan Xie Zhichao Sun;R.M.Saravana Kumar;Jisheng Li;Guiming Yang;Yan Xie

作者机构：Hebei Universities R&D Center for Sericulture and Specialty Enabling TechnologiesChengde 067000China Sericultural Research InsituteChengde Medical UniversityChengde 067000China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2022年第8卷第2期

页      面：184-194页

核心收录：

学科分类：090504[农学-特种经济动物饲养（含：蚕、蜂等）] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by the Natural Science Foundation of Hebei Province(Grant No.C2019406113) Hebei Provincial Department of Education(Grant No.QN2020236) Chengde Medical College Youth Foundation(Grant No.201913) 

主　　题：Mulberry miR171 qRT-PCR SCL Arabidopsis Stress 

摘      要：miR171 belongs to a highly conserved and ubiquitously expressed micro RNA gene family across species that play a critical role in controlling plant growth and development through the regulation of the miR171-SCL(scarecrow-like proteins)*** is limited research available on the evolutionary relationship and functional diversification of miR171 *** this study,we identified eight miR171 genes in the mulberry genome by bioinformatics analysis that were subsequently used to compare the evolutionary levels and explore abiotic stress mechanisms mediated by mno-miR171s(Morus notabilis miR171s).The results of phylogenetic analysis showed that the mature mno-miR171 sequences have strong sequence conservation,but their critical sites also exhibit high variation leading to functional *** quantitative real-time PCR,the expression profile of each mno-miR171 was analyzed under different stress *** mno-miR171s,apart from mnomiR171h,were found to be significantly up-regulated under salt and drought stress *** target genes of mno-miR171a namely,Morus020885 and Morus011800,were predicted and verified using the plural RNA method.5-rapid amplification of complementary DNA ends assays further to reveal that the target genes could be degraded by mno-miR171a *** of mno-miR171a in Arabidopsis improved the percentage of seed germination when the seeds were grown in NaCl-and mannitol-containing *** plants were observed to grow better under drought *** expression of various stress genes was significantly higher in transgenic plants than in wild type,except *** together,our study confirmed that mno-miR171a enhanced plant resistance to adverse stress environments via the regulation of the SCL targets.