Effects of Storage Temperature on the Effective Activity of Straw Frozen Semen after Thawing

作     者：Qiao Limin Ma Jianmin Qiao Fuqiang Yao Hua Xiao Xishan Hou Yinxu Fu Jingtao Ren Kang 

作者机构：Beijing Vocational College of Agriculture Technology Industry Group.BUA Animal Science and Technology CollegeBeijing University of Agriculture Beijing Municipal Bureau of Agriculture 

出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)

年 卷 期：2015年第7卷第4期

页      面：203-204,207页

学科分类：08[工学] 0905[农学-畜牧学] 09[农学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖] 

基　　金：Supported by the Technology Research and Demonstrational Popularization Project of Beijing Vocational College of Agriculture(XY-YF-15-07)(XY-YF-14-21) 

主　　题：Straw frozen semen Thawing temperature Thawing time Storage temperature Sperm motility 

摘      要：The aim was to discuss the optimal storage environment and proper insemination time after thawing of 0.25 mL straw frozen semen. Straw frozen semen was thawed at 40 ℃ for 20 s, and then stored at 0 -4 ℃, 14 - 16 ℃, 25 -27 ℃ for 2, 4, 6, 8 and 10 h, respectively. The sperm motility was detected. After thawing, semen was stored at 0 - 4 ℃ and 14 - 16 ℃ for 10 h. Their sperm motilities （0.434 ±0. 016 7 and 0.423 ±0.019 6） had no significant differences （P 〉 0.05） with initial thawing motility （0.441 ± 0.030）. Sperm motility reduced as the storage time prolonged at 25 -27 ℃. Sperm motility after 6 h had signifi- cant differences with that of initial thawing motility （P 〈 O. 05 ）, and sperm motilities after 8 and 10 h showed extremely significant differences （P 〈 0.01 ）. Thus, sperm motility after thawing was still very high after stored at 0 -4 ℃ and 14 - 16 ℃ within 10 h, which met the requirements for insemination. Under this temperature and time ranges, sperm could be carried over long distances, which had small effects on sperm quality and reached the expected insemination effects. However, under the temperature of 25 - 27 ℃, semen should be used for insemination within 6 h after thawing.