Overexpression of chaperonin containing TCP1, subunit 3 predicts poor prognosis in hepatocellular carcinoma

作     者：Xiao Cui Zhi-Ping Hu Zhao Li Peng-Ji Gao Ji-Ye Zhu 

作者机构：Department of Hepatobiliary Surgery Peking University People’s Hospital Beijing Key Surgical Basic Research Laboratory of Liver Cirrhosis and Liver Cancer Peking University People’s Hospital 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2015年第21卷第28期

页      面：8588-8604页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by Beijing Key Laboratory Special Fund No.Z141107004414042 

主　　题：Hepatocellular carcinoma Chaperonin ContainingTCP1, Subunit 3 Cell growth Invasion Prognosis 

摘      要：AIM:To investigate the value of chaperonin containing TCP1,subunit 3(CCT3) to predict the prognosis of patients with hepatocellular carcinoma(HCC) and determine its function in HCC ***:CCT3 expression levels were examined in human non-cancerous liver tissues and a variety of HCC cell lines by quantitative real-time PCR and ***3 expression was suppressed by small interfering *** effects of reducing CCT3 expression in HCC cells were ***3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide(MTT) assay,cell counting experiment,cell cycle assay,apoptosis assay and invasion assay were employed to evaluate cell functions in *** was performed on HCC *** addition,CCT3 expression in HCC specimens was also assessed at the protein and mRNA *** between clinicopathological characteristics and prognosis were analyzed,along with the possible mechanisms involved in CCT3 s function in HCC ***:The expression levels of CCT3 mRNA and protein were upregulated in HCC cell lines in contrast to adjacent non-cancerous *** CCT3 expression not only suppressed cell proliferation in cell counts,MTT assay,cell cycle assay and induced cell apoptosis(P 0.05 vs negative control),but also inhibited the tumor cell invasion capacity in vitro {P 0.01 vs negative control).Overexpression of CCT3 in the nuclei of cancer cells in HCC specimens(58of 104 patients,55.8%) was associated with poor prognosis in HCC patients(3-year survival rate,55.5%vs 84.2%,P = 0.020) after *** analyses showed that signal transducer and activator of transcription 3(STAT3) activation was decreased even when stimulated by interleukin-6 after knocking down CCT3 in the HepG2 cell ***:Overexpression of CCT3 in the nuclei of cancerous cells is associated with HCC ***3 may be a target that affects the activation of STAT3 in HCC.