Hormonal Regulation of Tissue-type Plasminogen Activator and Plasminogen Activator Inhibitor Type-1 Gene Expression in Cultured Mouse Sertoli Cells

作     者：刘以训 杜群 胡召元 Ny Tor 

作者机构：Department of Endocrinology Institute of Zoology Academia Sinica Beijing 100080 PRC State Key Laboratory of Reproductive Biology Institute of Zoology Academia Sinica Beijing 100080 PRC Unit for Applied Cell and Molecular Biology Umea University S90187 Umea Sweden 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：1993年第36卷第3期

页      面：319-328页

核心收录：

学科分类：0810[工学-信息与通信工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0708[理学-地球物理学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0704[理学-天文学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：Project supported by the National Natural Science Foundation of China and Swedish Medical Research Council 

主　　题：Sertoli cells tPA PAl-1 spermiation spermatogenesis. 

摘      要：We have demonstrated for the first time that (ⅰ) mouse Sertoli cells predominantly secrete tPA under the action of FSH and cAMP-generating agents, whereas Leydig cells mainly produce uPA; (ⅱ) Sertoli cells are also capable of secreting PAI-1, as well as FSH, growth factors and GnRH increase PAI-1 gene expression; (ⅲ) the increases in tPA and PAI-1 activities by different hormones in the conditioned media of Sertoli cells correspond to the increases in the levels of tPA and PAI-1 mRNA in the cultured cells, suggesting that the synthesis of the activator-inhibitor in mouse Sertoli cells is regulated at a transcriptional level and the tPA secreted by Sertoli cells may be involved in the spermatogenesis.