RNA Interference-based Suppression of Phosphoenolpyruvate Carboxylase Results in Susceptibility of Rapeseed to Osmotic Stress

作     者：Mei Chen Yunlai Tang Jingmei Zhang Mingfeng Yang Yinong Xu 

作者机构：Key Laboratory of Photobiology Instiiute of Botany Chinese Academy of Sciences Beijing 100093 China College of Life Science and Engineering Southwest University of Science and Technology Mianyang 621010 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2010年第52卷第6期

页      面：585-592页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：supported by the National Natural Science Foundation of China(30770224) 

主　　题：C3 plants Plants Planting Rapeseed Transgenic plants 

摘      要：The diverse functions of phosphoenolpyruvate carboxylase （PEPCase; EC 4.***.1.31） in C3 plants are not as well understood as in C4 plants. To investigate the functions of PEPCase in C3 plants, rapeseed （Brassica napus L.） PEPCase gene （referred to as BNPE15） was silenced by the RNA interference （RNAi） technique. Under normal growth conditions, no significant difference in lipid content and fatty acid composition were found between wild-type （WT） and transgenic rapeseed plants. However, when these plants were subjected to osmotic stress induced by osmoticum polyethylene glycol （PEG-6000）, membrane permeability and membrane lipid peroxidization in roots and leaves of transgenic plants were higher than those of WT plants. It suggested that transgenic plants are more susceptible to osmotic stress than WT plants. Taken together, the results showed that the suppression of PEPCase by RNAi leads to susceptibility to osmotic stress in rapeseed, and PEPCase is involved in the response of C3 plants to environmental stress.