Potential roles of vitamin D binding protein in attenuating liver injury in sepsis

作     者：Kun Xiao Du-Chao Zhang Ye Hu Li-Cheng Song Jian-Qiao Xu Wan-Xue He Pan Pan Yu-Wei Wang Li-Xin Xie Kun Xiao;Du?Chao Zhang;Ye Hu;Li?Cheng Song;Jian?Qiao Xu;Wan?Xue He;Pan Pan;Yu?Wei Wang;Li?Xin Xie

作者机构：Center of Pulmonary and Critical Care MedicineChinese People's Liberation Army(PLA)General HospitalNo.28Fuxing StreetHaidian DistrictBeijing 100853China Department of Pulmonary and Critical Care MedicineBeijing Shijitan HospitalBeijing 100071China Medical School of Chinese People's Liberation Army(PLA)Chinese PLA General HospitalBeijing 100853China Department of Geriatric Comprehensive Surgerythe Second Medical Center and National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalNo.28Fuxing StreetHaidian DistrictBeijing 100853China 

出 版 物：《Military Medical Research》 (军事医学研究（英文版）)

年 卷 期：2022年第9卷第5期

页      面：543-552页

核心收录：

学科分类：100218[医学-急诊医学] 1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：the Clinical Research Support Fund of Chinese PLA General Hospital(2018FC-WJFWZX-1-03) Youth Talents Promotion Project of China(17-JCJQ-QT-036) Natural Science Foundation of Beijing(7214254) 

主　　题：Vitamin D binding protein Sepsis Human Mouse Liver Injury c-Jun N-terminal kinase 

摘      要：Background:In sepsis,vitamin D binding protein(VDBP)has been shown to be *** current study examined the relationship between serum VDBP level and liver injury in sepsis patients,as well as in a mouse model for sepsis and in cultured liver epithelial cell line exposed to lipopolysaccharide(LPS).Methods:The human study included 78 sepsis patients and 50 healthy *** patients were categorized into sepsis survivor group(n=43)and sepsis non-survivor group(n=35)based on 28-day mortality for data *** male C57BL/6 mice were subjected to cecal ligation and puncture(CLP).Serum samples were collected on day 1,3,5 and 7 to determine the levels of VDBP,25-hydroxyvitamin D[25(OH)D_(3)],1,25-dihydroxyvitamin D[1,25(OH)_(2)D_(3)],interleukin-6(IL-6)and tumor necrosis factor alpha(TNF-α).Potential protective effects of VDBP overexpression against LPS-induced liver damage were examined in cultured THLE2 ***:Serum levels of VDBP,25(OH)D_(3),and 1,25(OH)_(2)D_(3)were significantly lower in sepsis patients *** healthy control(P0.001),as well as in the sepsis non-survivor group *** sepsis survivor group(P0.001,P=0.0338,or P=0.0013,respectively).Lower serum VDBP level was associated with higher Acute Physiology and Chronic Health Evaluation(APACHE)II score(r=−0.2565,P=0.0234)and Sequential Organ Failure Assessment score(r=−0.3522,P=0.0016),but lower serum albumin(ALB,r=0.4628,P0.001)and total protein(TP,r=0.263,P=0.02).In CLP mice,there was a 5-day period of serum VDBP reduction,followed by return towards the baseline on day *** was also decreased in LPS-treated THLE2 cells(P0.001).VDBP overexpression reduced LPS-induced THLE2 *** damage was associated with decreased oxidative stress and inactivation of the c-Jun N-terminal kinase signaling ***:VDBP may be protective against sepsis-induced liver injury.