Ecdysis triggering hormone peptide in the African malaria mosquito Anopheles gambiae:The peptide structure for receptor activation

作     者：Hawa Dembele Moritz Mating Rupinder Singh Soheila Fatehi Alvaro I.Herrera Yoonseong Park Om Prakash Hawa Dembele;Moritz Mating;Rupinder Singh;Soheila Fatehi;Alvaro I.Herrera;Yoonseong Park;Om Prakash

作者机构：Department of Biochemistry and Molecular BiophysicsKansas State UniversityManhattanKansas66506 Department of EntomologyKansas State UniversityManhattanKansas66506 Department of Chemistry&BiochemistryAuburn UniversityAuburnAlabamaUSA 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2022年第29卷第5期

页      面：1309-1317页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by National Institutes of Health(NIH)grants NIH-NIAID R21 AI135457 and AI163423,1S10OD026726 and USDA-NIFA,GRANT13066347 thank the Terry Johnson Cancer Research Center of Kansas State University and K-INBRE(NIH Grant P20GM103418)programs for supporting NMR studies.Contribution number 22-200-J from the Kansas Agricultural Experiment Station 

主　　题：Anopheles gambiae ecdysis triggering hormone G protein-coupled receptor insect malaria neuropeptide NMR spectroscopy 

摘      要：Infections by mosquito-borne diseases represent one of the leading causes of death in third world *** rapid progression of resistance to conventional insecticide causes a significant threat to the highly efficient preventive methods currently in *** neuropeptidergic system offers potential targets to control the insect *** essential roles of the neuropeptide ecdysis triggering hormone(ETH)in insect development and reproduction led us to attempt understanding of the fundamentals of the biochemical interaction between ETH and its receptor in the African malaria mosquito Anopheles *** of two ETH peptides of the African malaria mosquito(AgETH1),a small peptide hormone with 17 amino acid residues(SESPGFFIKLSKSVPRI-NH2),was studied to elucidate its molecular structure.N-termini deletions and mutations of conserved amino acids in the ligand revealed the critical residues for the receptor *** solution structure of AgETH1 using 2D 1H-1H nuclear magnetic resonance(NMR)spectroscopy and nuclear overhauser effect(NOE)derived constraints revealed a short alpha helix between residues 3S and *** NMR solution structure of AgETH1 will be of significant assistance for designing a new class of insecticidal compounds that acts on the AgETH receptor aiming for in silico docking studies.