The CCAAT/Enhancer Binding Protein Beta(cebpb)is essential for the development of enveloping layer(EVL)in zebrafish

作     者：Xin Zhang Zhichao Wu Mengdi Bu Ruiqin Hu Xiaowen Zhang Wenhao Li Liangbiao Chen 

作者机构：Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Ministry of Education)and International Research Center for Marine Biosciences(Ministry of Science and Technology)Shanghai Ocean UniversityShanghai201306China Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdao266237China 

出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)

年 卷 期：2023年第8卷第3期

页      面：252-260页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：supported by National Key Research and Development Program of China[2018YFD0900601] L.Chen,the open project fund from Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science(OF2019NO01) Doctor Fund of Shanghai Ocean University(A2-2006-21-200304) Science and Technology Development Fund of Shanghai Ocean University(A2-2006-20-200201)to W.Li 

主　　题：Cebpb Single-cell RNA sequencing Zebrafish Enveloping layer(EVL) 

摘      要：Epiboly,the coordinated spreading of the enveloping layer(EVL),the yolk syncytial layer(YSL)and the deep cells(DCs)toward the vegetal pole,is required for *** with the EVL leads to early developmental ***,the genetic factors underlying EVL differentiation are so far *** in-depth analyzing of single-cell sequencing data from 4 h post fertilization zebrafish embryos,we identified 105 genes(FDR0.05)which showed highly specific EVL expression,among which the transcription factor cebpb and genes(krt18,cldne,cldnb,krt4,krt5)involved in cell-cell junction formation were exclusively expressed in *** explore the role of cebpb in EVL differentiation,we knocked down its expression by morpholino-labeled antisense *** morphant embryos showed disrupted EVL and developmental termination prior to *** accordance,expression of the EVL specific cell-adhesion molecule genes were significantly ***,cebpb is the earliest EVL marker and essential for EVL differentiation in *** elucidation of the specific EVL gene set provides us new insights into the molecular mechanisms underlying EVL differentiation.