Screening and characterization of proteases produced by deep-sea cold seep bacteria

作     者：Chenchen GUO Chaomin SUN Shimei WU Chenchen GUO;Chaomin SUN;Shimei WU

作者机构：College of Life SciencesQingdao UniversityQingdao 266071China CAS Key Laboratory of Experimental Marine BiologyInstitute of OceanologyChinese Academy of SciencesQingdao 266071China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2022年第40卷第2期

页      面：678-689页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 0707[理学-海洋科学] 

基　　金：Supported by the National Natural Science Foundation of China(No.31600035)to Shimei WU the China Ocean Mineral Resources R&D Association Grant(No.DY135-B2-14)to Chaomin SUN。 

主　　题：deep-sea Bacillus alkaline protease purification characterization 

摘      要：Fifty protease-producing strains were screened from sediment of deep-sea cold seep,and divided into four diff erent categories:Bacillus,Pseudoalteromonas,Vibrio,and Alteromonas according to the sequences of 16s rRNA.Their abilities to produce protease,amylase,and lipase were determined,and a Bacillus strain gcc-1 displayed very strong alkaline protease activity and stability under different thermal and acidic conditions.The purifi cation of the protease produced by strain gcc-1 was carried out by precipitation with ammonium sulfate,and sequentially chromatographed by anion exchange column and gel filtration.The purifi ed protease showed a single band at the molecular weight of 28 kDa by SDS-PAGE.The characterization results show that the purified protease exhibited a considerable activity and stability in a wide thermal range of 10-80℃and a wide acidic range of pH 6.5-11.5,and displayed highest activity at 40℃ and pH 8.5.Notably,the protease still maintained high activity even at low to 10℃.Furthermore,the protease exhibited good stability in presence of diff erent surfactants,organic solvents,oxidizing agent H_(2)O_(2),and commercial detergents.Therefore,the protease produced by gcc-1 is a cold active and high stable enzyme,and has a promising potential in laundry detergent as an additive.