Lowered water table causes species substitution while nitrogen amendment causes species loss in alpine wetland microbial communities

作     者：Yuntao LI Jin-Sheng HE Hao WANG Jizhong ZHOU Yunfeng YANG Haiyan CHU Yuntao LI;Jin-Sheng HE;Hao WANG;Jizhong ZHOU;Yunfeng YANG;Haiyan CHU

作者机构：State Key Laboratory of Soil and Sustainable AgricultureInstitute of Soil ScienceChinese Academy of Sciences71 East Beijing RoadNanjing 210008(China) University of Chinese Academy of SciencesBeijing 100049(China) Department of EcologyCollege of Urban and Environmental SciencesPeking UniversityBeijing 100871(China) State Key Laboratory of Grassland Agro-EcosystemsCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000(China) State Key Joint Laboratory of Environment Simulation and Pollution ControlSchool of EnvironmentTsinghua UniversityBeijing 100084(China) Institute for Environmental GenomicsDepartment of Microbiology and Plant Biology and School of Civil Engineering and Environmental SciencesUniversity of OklahomaNorman OK 73019(USA) Earth Science DivisionLawrence Berkeley National LaboratoryBerkeley CA 94270(USA) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2021年第31卷第6期

页      面：912-922页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0818[工学-地质资源与地质工程] 0903[农学-农业资源与环境] 0901[农学-作物学] 071005[理学-微生物学] 

主　　题：alpha-diversity bacterial community beta-diversity microbial functional community N amendment water table lowering 

摘      要：Alpine wetlands are hotspots of carbon(C)storage and methane emission,and they could be key contributors to global warming.In recent years,rapid warming has lowered the water table in alpine wetlands on the Tibetan Plateau,concurrent with intensified nitrogen(N)deposition via anthropogenic activities.We carried out a field experiment to investigate the ecological impacts of these two factors on soil bacterial and functional communities,which are essential drivers of greenhouse gas emissions.Nitrogen amendment alone decreased the phylogenetic alpha-diversity of bacterial communities which could be offset by lowered water table.In contrast,microbial functional alpha-diversity,revealed by a high-throughput microarray,remained unchanged.Both bacterial and functional beta-diversity responded to lowered water table,but only bacterial community responded to N amendment.The alpha-Proteobacteria,beta-Proteobacteria,and Bacteroidetes were the major responsive bacterial lineages,and C degradation,methanogenesis,alkaline shock,and phosphorus oxidation were the major responsive functional processes.Partitioning analysis revealed that N amendment changed bacterial community structure mainly via species loss processes but did not affect bacterial functional communities,with soil pH and ammonium as the key factors influencing changes in bacterial community structure.Conversely,lowered water table altered bacterial and functional communities through species substitution processes linked to soil pH and soil moisture.According to our results,the response mechanisms of microbial communities to lowered water table and N amendment are fundamentally different in alpine wetlands.