Evolving strategies for marine enzyme engineering:recent advances on the molecular modification of alginate lyase
作者机构：College of Food Science and Light IndustryNanjing Tech UniversityNanjing211816China School of Medicine and Holistic Integrative MedicineNanjing University of Chinese MedicineNanjing210023China
出 版 物：《Marine Life Science & Technology》 (海洋生命科学与技术（英文）)
年 卷 期：2022年第4卷第1期
页 面：106-116页
核心收录：
学科分类：08[工学] 0824[工学-船舶与海洋工程]
基 金：supported by the National Natural Science Foundation of China(31601410) The Suqian City Science and Technology Project(L201906) the Postgraduate Research and Practice Innovation Program of Jiangsu Province(KYCX20_1103)
主 题：Alginate lyase Structure Catalytic mechanism Molecular modification Application
摘 要：Alginate,an acidic polysaccharide,is formed byβ-D-mannuronate(M)andα-L-guluronate(G).As a type of polysaccharide lyase,alginate lyase can efficiently degrade alginate into alginate oligosaccharides,having potential applications in the food,medicine,and agriculture ***,the application of alginate lyase has been limited due to its low catalytic efficiency and poor temperature *** recent years,various structural features of alginate lyase have been determined,resulting in modification strategies that can increase the applicability of alginate lyase,making it important to summarize and discuss the current *** this review,we summarized the structural features and catalytic mechanisms of alginate *** modification strategies,such as rational design,directed evolution,conserved domain recombination,and non-catalytic domain truncation,are also described in ***,the application of alginate lyase is *** comprehensive summary can inform future applications of alginate lyases.
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