The role of senescence-associated gene101 (PagSAG101a) in the regulation of secondary xylem formation in poplar

作     者：Hui He Xue-Qin Song Cheng Jiang Ying-Li Liu Dian Wang Shuang-Shuang Wen Guo-Hua Chai Shu-Tang Zhao Meng-Zhu Lu Hui He;Xue-Qin Song;Cheng Jiang;Ying-Li Liu;Dian Wang;Shuang-Shuang Wen;Guo-Hua Chai;Shu-Tang Zhao;Meng-Zhu Lu

作者机构：State Key Laboratory of Tree Genetics and BreedingResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China State Key Laboratory of Subtropical SilvicultureCollege of Forestry and BiotechnologyZhejiang A&F UniversityHangzhou 311300China College of AgronomyQingdao Agricultural UniversityQingdao 266109China College of Resources and EnvironmentQingdao Agricultural UniversityQingdao 266109China Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing 210037China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第1期

页      面：73-86页

核心收录：

学科分类：0907[农学-林学] 08[工学] 0829[工学-林业工程] 09[农学] 

基　　金：supported by the Fundamental Research Funds of CAF(CAFYBB2017ZY001) the Ten-thousand Talents Program of China to Meng-Zhu Lu 

主　　题：cambium PagSAG101a Populus secondary cell wall xylem formation 

摘      要：Wood is produced by the accumulation of secondary xylem via proliferation and differentiation of the cambium cells in woody plants. Identifying the regulators involved in this process remains a challenging task. In this study, we isolated Pag SAG101 a,the homolog of Arabidopsis thaliana SAG101, from a hybrid poplar(Populus alba × Populus glandulosa)clone 84 K and investigated its role in secondary xylem development. Pag SAG101 a was expressed predominantly in lignified stems and localized in thenucleus. Compared with non-transgenic 84 K plants,transgenic plants overexpressing Pag SAG101 a displayed increased plant height, internode number,stem diameter, xylem width, and secondary cell wall thickness, while opposite phenotypes were observed for Pag SAG101 a knock-out *** analyses revealed that differentially expressed genes were enriched for those controlling cambium cell division activity and subsequent secondary cell wall deposition during xylem *** addition, the tandem CCCH zinc finger protein Pag C3 H17, which positively regulates secondary xylem width and secondary wall thickening in poplar, could bind to the promoter of Pag SAG101 a and mediate the regulation of xylem *** results support that Pag SAG101 a, downstream of Pag C3 H17, functions in wood development.