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Journal of Integrative Plant Biology 2024年第1期66卷 86-102页

作者： Yufei Zhang Lingyan Wang Yuexin Wu Donghui Wang Xin‐Qiang He State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China

Secondary vascular tissue(SVT)development and regeneration are regulated by phytohormones.In this study,we used an in vitro SVT regeneration system to demonstrate that gibberellin(GA)treatment significantly promotes auxin-induced cambium reestablishment.Altering GA content by overexpressing or knocking down ent-kaurene synthase(KS)affected secondary growth and SVT regeneration in poplar.The poplar DELLA gene GIBBERELLIC ACID INSENSITIVE(PtoGAI)is expressed in a specific pattern during secondary growth and cambium regeneration after girdling.Overexpression of PtoGAI disrupted poplar growth and inhibited cambium regeneration,and the inhibition of cambium regeneration could be partially restored by GA application.Further analysis of the PtaDR5:GUS transgenic plants,the localization of PIN-FORMED 1(PIN1)and the expression of auxin-related genes found that an additional GA treatment could enhance the auxin response as well as the expression of PIN1,which mediates auxin transport during SVT regeneration.Taken together,these findings suggest that GA promotes cambium regeneration by stimulating auxin signal transduction.

关键词： auxin cambium gibberellin populus secondary vascular tissue regeneration

The super-pangenome of populus unveils genomic facets for its adaptation and diversification in widespread forest trees

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Molecular Plant 2024年第5期17卷 725-746页

作者： Tingting Shi Xinxin Zhang Yukang Hou Changfu Jia Xuming Dan Yulin Zhang Yuanzhong Jiang Qiang Lai Jiajun Feng Jianju Feng Tao Ma Jiali Wu Shuyu Liu Lei Zhang Zhiqin Long Liyang Chen Nathaniel R.Street Par K.Ingvarsson Jianquan Liu Tongming Yin Jing Wang Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education College of Life SciencesSichuan UniversityChengduSichuanChina College of Horticulture and Forestry Tarim UniversityAlar 843300China Umea Plant Science Centre Department of Plant PhysiologyUmea UniversityUmeaVasterbottenSweden Linnean Centre for Plant Biology Department of Plant BiologyUppsala BioCenterSwedish University of Agricultural SciencesUppsalaSweden The Key Laboratory of Tree Genetics and Biotechnology of Jiangsu Province and Education Department of China Nanjing Forestry UniversityNanjingJiangsuChina

Understanding the underlying mechanisms and links between genome evolution and adaptive innovations stands as a key goal in evolutionary studies.Poplars,among the world’s most widely distributed and cultivated trees,exhibit extensive phenotypic diversity and environmental adaptability.In this study,we present a genus-level super-pangenome comprising 19 populus genomes,revealing the likely pivotal role of private genes in facilitating local environmental and climate adaptation.Through the integration of pangenomes with transcriptomes,methylomes,and chromatin accessibility mapping,we unveil that the evolutionary trajectories of pangenes and duplicated genes are closely linked to local genomic landscapes of regulatory and epigenetic architectures,notably CG methylation in gene-body regions.Further comparative genomic analyses have enabled the identification of 142202 structural variants across species that intersect with a significant number of genes and contribute substantially to both phenotypic and adaptive divergence.We have experimentally validated a∼180-bp presence/absence variant affecting the expression of the CUC2 gene,crucial for leaf serration formation.Finally,we developed a user-friendly web-based tool encompassing the multi-omics resources associated with the populus super-pangenome(http://***).Together,the present pioneering super-pangenome resource in forest trees not only aids in the advancement of breeding efforts of this globally important tree genus but also offers valuable insights into potential avenues for comprehending tree biology.

关键词： populus pangenomes whole-genome duplication structural variation genome evolution

Wood of trees:Cellular structure,molecular formation,and genetic engineering

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Journal of Integrative Plant Biology 2024年第3期66卷 443-467页

作者： Yingying Zhu Laigeng Li State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems and College of Ecology Lanzhou UniversityLanzhou 730000China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China

Wood is an invaluable asset to human society due to its renewable nature,making it suitable for both sustainable energy production and material manufacturing.Additionally,wood derived from forest trees plays a crucial role in sequestering a significant portion of the carbon dioxide fixed during photosynthesis by terrestrial plants.Nevertheless,with the expansion of the global population and ongoing industrialization,forest coverage has been substantially decreased,resulting in significant challenges for wood production and supply.Wood production practices have changed away from natural forests toward plantation forests.Thus,understanding the underlying genetic mechanisms of wood formation is the foundation for developing high-quality,fast-growing plantation trees.Breeding ideal forest trees for wood production using genetic technologies has attracted the interest of many.Tremendous studies have been carried out in recent years on the molecular,genetic,and cell-biological mechanisms of wood formation,and considerable progress and findings have been achieved.These studies and findings indicate enormous possibilities and prospects for tree improvement.This review will outline and assess the cellular and molecular mechanisms of wood formation,as well as studies on genetically improving forest trees,and address future development prospects.

关键词： cambium cell wall populus wood formation xylem

Fine-tuning brassinosteroid biosynthesis via 3′UTR-dependent decay of CPD mRNA modulates wood formation in populus

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Journal of Integrative Plant Biology 2023年第8期65卷 1852-1858页

作者： Dian Wang Xiaoning Hao Li Xu Mengyan Zhao Congpeng Wang Xihao Yu Yingzhen Kong Mengzhu Lu Gongke Zhou Guohua Chai Xianfeng Tang College of Agronomy Qingdao Agricultural UniversityQingdao 266109China College of Resources and Environment Qingdao Agricultural UniversityQingdao 266109China College of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdao 266109China State Key Laboratory of Subtropical Silviculture School of Forestry and BiotechnologyZhejiang A&F UniversityHangzhou311300China

Brassinosteroids(BRs)are plant hormones that regulate wood formation in trees.Currently,little is known about the post-transcriptional regulation of BR synthesis.Here,we show that during wood formation,fine-tuning BR synthesis requires 3′UTR-dependent decay of populus CONSTITUTIVE PHOTOMORPHOGENIC DWARF 1(PdCPD1).Overexpression of PdCPD1 or its 3′UTR fragment resulted in a significant increase of BR levels and inhibited secondary growth.In contrast,transgenic poplars repressing PdCPD13′UTR expression displayed moderate levels of BR and promoted wood formation.We show that the populus GLYCINE-RICH RNA-BINDING PROTEIN1(PdGRP1)directly binds to a GU-rich element in 3′UTR of Pd CPD1,leading to its mRNA decay.We thus provide a post-transcriptional mechanism underlying BRs synthesis during wood formation,which may be useful for genetic manipulation of wood biomass in trees.

关键词： Brassinsteroids biosynthesis CPD populus RNABINDING PROTEIN 3'UTR regulation

Conservation and Diversity of MicroRNA-associated Copper-regulatory Networks in populus trichocarpa

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Journal of Integrative Plant Biology 2011年第11期53卷 879-891页

作者： Shanfa Lu Chenmin Yang Vincent L. Chiang Medicinal Plant Cul＇tivation Research Center Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193 China Forest Biotechnology Group Department of Forestry and Environmental Resources College of Natural Resources North Carolina State University Raleigh NC 27695 USA

Plants develop important regulatory networks to adapt to the frequently-changing availability of copper （Cu）, However, little is known about miRNA-associated Cu-regulatory networks in plant species other than Arabidopsis. Here, we report that Cu-responsive miRNAs in populus trichocarpa （Torr. ＆ Gray） include not only conserved miR397, miR398 and miR408, but also populus-specific miR1444, suggesting the conservation and diversity of Cu-responsive miRNAs in plants. Copper-associated suppression of mature miRNAs is in company with the up-regulation of their target genes encoding Cu-containing proteins in populus. The targets include miR397-targeted PtLAC5, PtLAC6 and PtLAC110a, miR398- targeted PtCSD1, PtCSD2a and PtCSD2b, miR408-targeted PtPCL1, PtPCL2, PtPCL3 and PtLAC4, and miR1444-targeted PtPP03 and PtPP06. Consistently, P. trichocarpa miR408 promoter-directed GUS gene expression is down-regulated by Cu in transgenic tobacco plants. Cu-response elements （CuREs） are found in the promoters of Cu-responsive miRNA genes. We identified 34 SQUAMOSA-promoter binding protein-like （SPL） genes, of which 17 are full-length PtSPL proteins or partial sequences with at least 300 amino acids. Phylogenetic analysis indicates that PtSPL3 and PtSPL4 are CuRE-binding proteins controlling Cu-responsive gene expression. Cu appears to be not involved in the regulation of these transcription factors because neither PtSPL3 nor PtSPL4 is Cu-regulated and no CuRE exists in their promoters.

关键词： copper Cu-response element microRNA populus SPB domain protein.

Pretreatment of populus tomentiglandulosa protects hippocampal CA1 pyramidal neurons from ischemia-reperfusion injury in gerbils via increasing SODs expressions and maintaining BDNF and IGF-I expressions

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Chinese Journal of Natural Medicines 2019年第6期17卷 424-434页

作者： Tae-Kyeong Lee Joon Ha Park Ji Hyeon Ahn Hyunjung Kim Minah Song Jae-Chul Lee Jong Dai Kim Yong Hwan Jeon Jung Hoon Choi Choong Hyun Lee In Koo Hwang YAN Bing-Chun Moo-Ho Won Il Jun Kang Department of Neurobiology School of MedicineKangwon National UniversityChuncheon 24341Republic of Korea Department of Biomedical Science Research Institute of Bioscience and BiotechnologyHallym UniversityChuncheon 24252Republic of Korea Division of Food Biotechnology School of BiotechnologyKangwon National UniversityChuncheon 24341Republic of Korea Department of Radiology School of MedicineKangwon National UniversityChuncheon 24289Republic of Korea Department of Anatomy College of Veterinary MedicineKangwon National UniversityChuncheon 24341Republic of Korea Department of Pharmacy College of PharmacyDankook UniversityCheonan 31116Republic of Korea Department of Anatomy and Cell Biology College of Veterinary Medicine and Research Institute for Veterinary ScienceSeoul National UniversitySeoul 08826Republic of Korea Jiangsu Key Laboratory of Integrated Traditional Chinese Western Medicine for Prevention and Treatment of Senile DiseasesYangzhou 225001China Department of Food Science and Nutrition Hallym UniversityChuncheon 24252Republic of Korea

To examine the effects of populus tomentiglandulosa(PT) extract on the expressions of antioxidant enzymes and neurotrophic factors in the cornu ammonis 1(CA1) region of the hippocampus at 5 min after inducing transient global cerebral ischemia(TGCI) in gerbils, TGCI was induced by occlusion of common carotid arteries for 5 min. Before ischemic surgery, 200 mg·kg–1 PT extract was orally administrated once daily for 7 d. We performed neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B staining. Furthermore, we determined in situ production of superoxide anion radical, expression levels of SOD1 and SOD2 as antioxidant enzymes and brain-derived neurotrophic factor(BDNF) and insulin-like growth factor I(IGF-I) as neurotrophic factors. Pretreatment with 200 mg·kg–1 PT extract prevented neuronal death(loss). Furthermore, pretreatment with 200 mg·kg–1 PT extract significantly inhibited the production of superoxide anion radical, increased expressions of SODs and maintained expressions of BDNF and IGF-I. Such increased expressions of SODs were maintained in the neurons after IRI. In summary, pretreated PT extract can significantly increase levels of SODs and protect the neurons against TGCI, suggesting that PT can be a useful natural agent to protect against TGCI.

关键词： Transient global cerebral ischemia populus tomentiglandulosa Neuroprotective effects Antioxidant enzymes

PagGRF12a interacts with PagGIF1b to regulate secondary xylem development through modulating PagXND1a expression in populus alba×P.glandulosa

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Journal of Integrative Plant Biology 2021年第10期63卷 1683-1694页

作者： Jinnan Wang Houjun Zhou Yanqiu Zhao Cheng Jiang Jihong Li Fang Tang Yingli Liu Shutang Zhao Jianjun Hu Xueqin Song MengZhu Lu State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of the State ForestryAdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Key Laboratory of Agricultural Ecology and Environment College of ForestryShandong Agricultural UniversityTai'an 271018China School of Agriculture Ludong UniversityYantai 264025China State Key Laboratory of Subtropical Silviculture School of Forestry and BiotechnologyZhejiang Agriculture&Forestry UniversityHangzhou 311300China CoInnovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037China

Growth-regulating factors(GRFs)are important regulators of plant development and growth,but their possible roles in xylem development in woody plants remain unclear.Here,we report that populus alba×Papulus glandulosa PagGRF12a negatively regulates xylem development in poplar.PagGRF12a is expressed in vascular tissues.Compared to non-transgenic control plants,transgenic poplar plants overexpressing PagGRF12a exhibited reduced xylem width and plants with repressed expression of PagGRF12a exhibited increased xylem width.Xylem NAC domain 1(XND1)encodes a NAC domain transcription factor that regulates xylem development and transcriptional analyses revealed that PagXND1a is highly upregulated in PagGRF12a-overexpressing plants and downregulated in PagGRF12a-suppressed plants,indicating that PagGRF12a may regulate xylem development through PagXND1a.Transient transcriptional assays and chromatin immunoprecipitation-polymerase chain reaction assays confirmed that PagGRF12a directly upregulates PagXND1a.In addition,PagGRF12a interacts with the GRF-Interacting Factor(GIF)PagGIF1b,and this interaction enhances the effects of PagGRF12a on PagXND1a.Our results indicate that PagGRF12a inhibits xylem development by upregulating the expression of PagXND1a.

关键词： GRF GIF1 populus wood formation XND1 xylem development

Phosphorylation of LTF1, an MYB Transcription Factor in populus, Acts as a Sensory Switch Regulating Lignin Biosynthesis in Wood Cells

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Molecular Plant 2019年第10期12卷 1325-1337页

作者： Jinshan Gui Laifu Luo Yu Zhong Jiayan Sun Toshiaki Umezawa Laigeng Li National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences Shanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China School of Life Science Lanzhou UniversityLanzhou 730000China Research Institute for Sustainable Humanosphere Kyoto UniversityUjiKyoto 611-0011Japan

Lignin is specifically deposited in plant secondary cell walls,and initiation of lignin biosynthesis is regulated by a variety of developmental and environmental signals.However,the mechanisms governing the regulation of lignin biosynthesis remain to be elucidated.In this study,we identified a lignin biosynthesis-associated transcription factor(LTF)from populus,LTF1,which binds the promoter of a key lignin biosynthetic gene encoding 4-coumarate-CoA ligase(4CL).We showed that LTF1 in its unphosphorylated state functions as a regulator restraining lignin biosynthesis.When LTF1 becomes phosphorylated by PdMPK6 in response to external stimuli such as wounding,it undergoes degradation through a proteasome pathway,resulting in activation of lignification.Expression of a phosphorylation-null mutant version of LTF1 led to stable protein accumulation and persistent attenuation of lignification in wood cells.Taken together,our study reveals a mechanism whereby LTF1 phosphorylation acts as a sensory switch to regulate lignin biosynthesis in response to environmental stimuli.The discovery of novel modulators and mechanisms modifying lignin biosynthesis has important implications for improving the utilization of cell-wall biomass.

关键词： lignin populus secondary xylem cell wall

Identification and Characterization of the populus AREB/ABF Subfamily

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Journal of Integrative Plant Biology 2013年第2期55卷 177-186页

作者： Lexiang Ji Jia Wang Meixia Ye Ying Li Bin Guo Zhong Chen Hao Li Xinmin An National Engineering Laboratory for Tree Breeding NDRC Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants MOE The Tree and Ornamental Plant Breeding and Biotechnology Laboratory SFA College of Biological Sciences and Biotechnology Beijing Forestry University

Abscisic acid （ABA） is a major plant hormone that plays an important role in responses to abiotic stresses. The ABA-responsive element binding protein/ABRE-binding factor （AREB/ABF） gene subfamily contains crucial transcription factors in the ABA-mediated signaling pathway. In this study, a total of 14 putative AREB/ABF members were identified in the populus trichocarpa Torr. ＆ Gray. genome using five AREB/ABF amino acid sequences from Arabidopsis thaliana L. as probes. The 14 putative populus subfamily members showed high protein similarities, especially in the basic leucine zipper （bZIP） domain region. A neighbor-joining analysis combined with gene structure data revealed homology among the 14 genes. The expression patterns of the populus AREB/ABF subfamily suggested that the most abundant transcripts of 11 genes occurred in leaf tissues, while two genes were most transcribed in root tissues. Significantly, eight populus AREB/ABF gene members were upregulated after treatment with 100 t^M exogenous ABA, while the other six members were downregulated. We identified the expression profiles of the subfamily members in populus tissues and elucidated different response patterns of populus AREB/ABF members to ABA stress. This study provided insight into the roles of populus AREB/ABF homologues in plant response to abiotic stresses.

关键词： Arabidopsis thafiana populus AREB/ABF bioinformatics qRT-PCR.

Drought stress tolerance analysis of populus ussuriensis clones with different ploidies

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Journal of Forestry Research 2019年第4期30卷 1267-1275页

作者： Jianqiu Xu Jiaojiao Jin Hui Zhao Kailong Li State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People's Republic of China College of Forestry Henan Agricultural UniversityZhengzhou 450002People's Republic of China

The selection of drought-tolerant plants is an important aspect of plant breeding.We studied physiological and biochemical mechanisms of different ploidies of populus ussuriensis Kom.that relate to drought stress tolerance.We used a 5%(v/v)polyethylene glycol(PEG-6000)solution to simulate drought stress.We recorded leaf phenotypes including color,dry area and curl degree.We evaluated sequential variations in some drought stress tolerance-related physiological and biochemical indices and compared these among diploid clones(CK),triploid clones(T12)and tetraploid clones(F20).T12 leaves exhibited slightly more drought stress damage than CK and F20 leaves.CK leaves suffered the most severe drought stress damage.The physiological and biochemical indices of the different ploidies differed significantly 12 days after drought stress treatment.The activities of superoxide dismutase,peroxidase,catalase and proline in the triploid(T12)leaves were the highest.The relative electric conductivity and malondialdehyde content of T12 leaves were the lowest.The index values of F20 were between those ofthe diploid and triploid.In consideration of these results,the drought resistance of the three different ploidies of P.ussuriensis can be ranked as T12>F20>CK.We speculate that the gene expression patterns of polyploid clones of poplar will change after genome doubling and that some of the drought stress tolerance-related physiological and biochemical indices will be improved,resulting in greater drought tolerance of polyploid clones.

关键词： populus ussuriensis Polyploidy Drought stress Tolerance