Development of bioorthogonal SERS imaging probe in biological and biomedical applications

作     者：Chonggui Qiu Ziyi Cheng Chuanzhu Lv Rui Wang Fabiao Yu Chonggui Qiu;Ziyi Cheng;Chuanzhu Lv;Rui Wang;Fabiao Yu

作者机构：The First Affiliated Hospital of Hainan Medical UniversityKey Laboratory of Emergency and TraumaMinistry of EducationKey Laboratory of Hainan Trauma and Disaster RescueInstitute of Functional Materials and Molecular ImagingCollege of PharmacyCollege of Emergency and TraumaHainan Medical UniversityHaikou 571199China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2021年第32卷第8期

页      面：2369-2379页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 08[工学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported by Hainan Provincial Natural Science Foundation of China (Nos.2019RC220,820RC641,2019RC210) National Natural Science Foundation of China (Nos.21864011,22064009,21775162) Hainan Key Research and Development Project (No.ZDYF2020133) CAMS Innovation Fund for Medical Sciences (No.2019-I2M-5-023) Nanhai Young-Talent Program of Hainan (No.20202007) Hundred Talent Program of Hainan (2018)。 

主　　题：Surface-enhanced Raman scattering(SERS) Bioorthogonal molecule Visualization Disease diagnosis Therapy 

摘      要：Living-cell imaging demands high specificity,sensitivity,and minimal background interference to the targets of interest.However,developing a desirable imaging probe that can possess all the above features is still challenging.The bioorthogonal surface-enhanced Raman scattering(SERS) imaging has been recently emerged through utilizing Raman reporters with characteristic peaks in Raman-silent region of cells(1800-2800 cm^(-1)),which opens a revolutionary avenue for living-cell imaging with multiplexing capability.In this review,we focus on the recent advances in the technology development and the biological and biomedical applications of the living-cell bioorthogonal SERS imaging technique.After introduction of fundamental principles for bioorthogonal tag or label,we present applications for visualization of various intracellular components and environment including proteins,nucleic acids,lipids,pH and hypoxia,even for cancer diagnosis in tissue samples.Then,various bioorthogonal SERS imaging-guided thera py strategies have been discussed such as photothera py and surge ry.In conclusion,this strategy has great potential to be a flexible and robust tool for visualization detection and diseases diagnosis.