The gut microbiome and metabolome of Himalayan Griffons(Gyps himalayensis):insights into the adaptation to carrion-feeding habits in avian scavengers

作     者：Wen Wang Xiaolong Gao Sisi Zheng Zhuoma Lancuo Ying Li Lilin Zhu Jianping Hou Jiayi Hai Xin Long Hanxi Chen Alexey Druzyaka Kirill Sharshov Wen Wang;Xiaolong Gao;Sisi Zheng;Zhuoma Lancuo;Ying Li;Lilin Zhu;Jianping Hou;Jiayi Hai;Xin Long;Hanxi Chen;Alexey Druzyaka;Kirill Sharshov

作者机构：State Key Laboratory of Plateau Ecology and AgricultureQinghai UniversityXi’ning 810016China College of Agriculture and Animal HusbandryQinghai UniversityXi’ning 810016China College of Finance and EconomicsQinghai UniversityXi’ning 810016China Xining Wild Animal Epidemic Disease Monitoring StationXi’ning 810001China College of Eco-Environmental EngineeringQinghai UniversityXi’ning 810016China Institute of Systematics and Ecology of AnimalsSiberian Branch of the Russian Academy of SciencesNovosibirsk 630091Russia Federal Research Center of Fundamental and Translational MedicineNovosibirsk 630117Russia. 

出 版 物：《Avian Research》 (鸟类学研究（英文版）)

年 卷 期：2021年第12卷第4期

页      面：682-698页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0905[农学-畜牧学] 0906[农学-兽医学] 0713[理学-生态学] 

基　　金：funded by the National Natural Science Foundation of China(Grant No.31960277) the National Natural Science Foundation of China and Russian Foundation for Basic Research Cooperative Exchange Project(Grant Nos.32111530018,21-54-53031) the Natural Science Foundation of Qinghai Province of China(Grant No.2018-ZJ-932Q) supported by“1000 Talent”programs of Qinghai Province 

主　　题：Avian scavengers Culture Microbiome Pathogens Vultures 

摘      要：Background:Himalayan Griffons(Gyps himalayensis),large scavenging raptors widely distributed in Qinghai-Tibetan Plateau,have evolved a remarkable ability to feed on carcasses without suffering any adverse *** gut microbiome plays an important role in animal physiological and pathological processes,and has also been found to play a health protective role in the vulture adaptation to ***,the microbial taxonomic diversity(including nonculturable and culturable microbes),functions,and metabolites related to Himalayan Griffons have not been fully ***:In the present study,the 28 fecal samples of the Himalayan Griffons and 8 carrion samples were collected and sequenced using high-throughput 16 S rRNA gene sequencing methods to analyze the composition and functional structures of the *** fecal samples of the Himalayan Griffons were analyzed using untargeted Liquid Chromatography Mass Spectroscopy(LC-MS)to identify *** used different culture conditions to grow Himalayan Griffons gut *** effects of gut beneficial bacteria on 5 common pathogenic bacteria were also tested using the Oxford cup ***:According to the results of the culture-independent method,a high abundance of four major phyla in Himalayan Griffons were identified,including Fusobacteria,Firmicutes,Bacteroidetes,and *** most abundant genera were Fusobacterium,followed by Clostridium_sensu_stricto_1,Cetobacterium,Epulopiscium,and *** predicted primary functional categories of the Himalayan Griffons gut microbiome were associated with carbohydrate and amino acid metabolism,replication and repair,and membrane ***-MS metabolomic analysis showed a total of 154 metabolites in all the fecal *** yielded 184 bacterial isolates with Escherichia coli,Enterococcus faecium,Enterococcus hirae,and Paeniclostridium sordellii as most common ***,7 potential beneficial gut bacteria