Chemerin deficiency regulates adipogenesis is depot different through TIMP1
作者机构：Biology Science InstitutesChongqing Medical UniversityChongqing400016PR China Center for Translational Research in Hematologic MalignanciesHouston Methodist Cancer CenterHouston Methodist Research InstituteHoustonTX 77030USA Key Laboratory of Metabolic Molecular MedicineThe Ministry of EducationDepartment of Biochemistry and Molecular BiologyFudan University Shanghai Medical CollegeShanghai200032PR China Shanghai Public Health Clinical CenterFudan UniversityShanghai201508PR China Department of ImmunologyFudan University Shanghai Medical CollegeShanghai200032PR China
出 版 物：《Genes & Diseases》 (基因与疾病（英文）)
年 卷 期：2021年第8卷第5期
页 面：698-708页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 0703[理学-化学] 10[医学]
基 金：Fund from Joint International Research Laboratory of Reproduction and Development,Institute of Life Science,Chongqing Medical University.National Key R&D Program of China[grant numbers 2018YFA0800401 to X.Li] National Natural Science Foundation of China[grant numbers 81770861 and 31571401 to X.Li] Chongqing Science and Technology Foundation[grant number cstc2018jcy-jAX0232] Science and Technology Research Program of Chongqing Municipal Education Commission[grant number KJZD-K201800402] The Outstanding Talent Fund of Chongqing Medical University[grant number BJRC201707] Chongqing education committee[grant number CYB17105].
主 题：Adipogenesis Adipose tissue depot Chemerin Inflammation TIMP1
摘 要：Adipocytes and immune cells are vital for the development of adipose tissue.Adi-pokines secreted by adipocytes regulate adipogenesis and body metabolism.Chemerin is one of the adipokines.However,the function and mechanism of chemerin in adipose tissue are not fully illuminated.Compared with wild type(WT)mice,Rarres2^(-/-)mice gained weight and significantly increased fat distribution in subcutaneous adipose tissue(SAT),rather than visceral adipose tissue(VAT)on high fat diet(HFD).PPARg and C/EBPa,the master regulators of adipogenesis,were up-regulated in SAT and down-regulated in VAT in Rarres2^(-/-)mice comparing with WT mice.Inspite of chemerin deficiency or not,the ratio of adipocyte-progenitors to total cells and the differentiation capacity of adipocyte-progenitors were similar in SAT and VAT,but macrophage infiltration in VAT was more severe than in SAT in Rarres2^(-/-)mice.Furthermore,CD45þimmune cells supernatant from Rarres2^(-/-)SAT pro-moted the differentiation of adipocyte-progenitors and 3T3-L1 cells.Adipokine array assay of CD45þimmune cells supernatant revealed that metalloproteinase inhibitor 1(TIMP1),an in-hibitor of adipogenesis,was reduced in Rarres2^(-/-)SAT,but increased in Rarres2^(-/-)VAT.As we specifically knocked down chemerin in SAT,TIMP1 was down-regulated and adipogenesis was promoted with reducing infiltration of macrophages.The present study demonstrates that the effects of chemerin on adipose tissue is depot different,and specific knock down chemerin in SAT promote adipogenesis and improve glucose tolerance test(GTT)and insulin tolerance test(ITT).This suggests a potential therapeutic target for chemerin in the treatment of obesity related metabolic disorder.
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